相关实验视频

Updated: May 28, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

探测和量化循环RNA变异的过去,现在和未来的策略

He Lin¹, Vanessa M Conn¹, Simon J Conn¹

¹Flinders Health and Medical Research Institute, College of Medicine & Public Health, Flinders University, Adelaide, Australia.

The FEBS journal

|February 11, 2025

概括

循环RNAs (circRNAs) 是使用先进测序检测到的独特RNA分子. 新的方法改善了它们在生物样本中的敏感检测和量化.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
在RNA生物学,RNA生物学.

背景情况:

循环RNAs (circRNAs) 是由背接形成的共关闭的RNA转录.
尽管存在无处不在,但由于其独特的结构,circRNAs在历史上很难被检测到.
最近已经确定了超过300万个独特的circRNA转录.

研究的目的:

审查检测和量化circRNAs方面的进展.
要突出下一代测序 (NGS) 方法用于circRNA变体检测.
讨论 circRNA量化准确的规范化方法.

主要方法:

审查关于circRNA检测和量化现有的文献.
专注于下一代测序 (NGS) 策略.
讨论生物信息学管道和长读序列的讨论.

主要成果:

循环RNA是特定于细胞类型的,稳定的,并且通常是保存的.
低表达水平和复杂的结构挑战了准确的检测.
对于全面的表征,NGS,丰富和专门的生物信息学至关重要.

结论:

特定和超敏感的检测方法对于circRNA研究至关重要.
测序技术的进步对于识别circRNA变异至关重要.
准确的量化和规范化是了解生物样本中circRNA功能的关键.

关键词:

替代性拼接是一种替代性的拼接.环绕RNAss 的时间.圆形的RNAs是圆形的RNAs.长阅读序列编排长阅读序列编排这是下一代测序.

更多相关视频

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...