一个近红外光探测器,具有很大的杆位转移,用于检测环境水中硫化,葡萄酒样品和生物系统中的硫化
Shuai Gong1, Yue Gu1, Jixiang Tian1
1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Journal of agricultural and food chemistry
|February 12, 2025
概括
一个新的近红外光探头NPD-H2S被开发用于敏感的硫化 (H2S) 检测. 这种探测器可以在环境样本中进行定量分析,便携式蒸汽检测,以及在细胞和斑马鱼中进行生物成像,帮助进行植物压力研究.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 硫化 (H2S) 是一个关键的分子,涉及各种生理过程,环境问题,食品安全和植物发展.
- 精确和灵敏地检测H2S对于监测它在各种生物和环境系统中的作用至关重要.
研究的目的:
- 开发一种基于诺皮的新型近红外光探针,用于敏感和选择性检测硫化 (H2S).
- 评估探测器在环境样本中H2S的定量分析及其在生物成像中的应用方面的性能.
主要方法:
- 一个新的基于诺皮的近红外光探针的合成和表征,被指定为NPD-H2S.
- 评估探头性能,包括检测极限,选择性和响应时间.
- 应用NPD-H2S用于水和葡萄酒中的定量H2S检测,制造便携式H2S蒸汽传感器,并在盐应激下对细胞 (HeLa,MCF-7),斑马鱼和*Arabidopsis thaliana*进行生物成像.
主要成果:
- 开发的探测器NPD-H2S表现出卓越的性能,具有较大的斯托克斯转移,低检测极限,良好的选择性和快速响应时间.
- 在环境水和葡萄酒样本中,NPD-H2S成功量化了H2S,并通过智能手机集成的纳米纤维膜实现了H2S蒸汽的敏感检测.
- 该探测器在各种细胞系,斑马鱼中促进了有效的H2S生物成像,并在盐应激期间监测了Arabidopsis thaliana的H2S上调.
结论:
- NPD-H2S探测器是监测环境和生物系统中H2S波动的高效工具.
- 这项工作提出了一个新的概念,用于在植物栽培中使用外部压力干预,以H2S生物成像为指导.
- 开发的探测器为各种H2S检测和成像应用提供了一个多功能平台.
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