通过泛基因组学识别和描述古人类的伪氨酸生物合成基因
Valérian Lupo1,2, Célyne Roomans1, Edmée Royen1
1InBioS-PhytoSYSTEMS, Eukaryotic Phylogenomics, University of Liège, Liège, Belgium.
mSystems
|February 12, 2025
概括
考古伪氨酸的合成依赖于来自细菌的木氨酸连接酶,这挑战了进化论的观点. 这项研究揭示了古老细胞壁生物合成中的关键酶的细菌起源.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 生物信息学是一种生物信息学.
背景情况:
- 펩티도글리칸 (PG) 是细菌细胞壁的组成部分,而古生物则利用各种聚合物,包括伪氨酸 (PM).
- 含有PM的古生物学物具有细菌PG生物合成基因的同类物,但它们的起源和功能仍然不清楚.
- 了解颗粒物生物合成对于开发抗古物剂至关重要,特别是针对机会性病原体,如甲生物菌.
研究的目的:
- 通过泛基因组方法识别参与古生物中的伪素 (PM) 生物合成的蛋白质.
- 研究这些蛋白质在古生物和细菌中的进化起源和关系.
- 识别可能参与PM合成的新型基因.
主要方法:
- 开发了一种泛基因生物信息管道,以识别古生物中的PM生物合成蛋白.
- 利用HMM序列挖掘和为Mur域含有,ATP抓取和MraY类家族的族系推断.
- 分析了候选蛋白质在古生物和细菌中的分类学分布和进化关系.
主要成果:
- 在PM生物合成中涉及的古老的muramyl连接酶是细菌起源的,通过横向基因转移和基因重复获得.
- ATP-grasp和MraY类家族的考古成员似乎并没有起源于细菌.
- 鉴定了五种可能参与PM合成的新候选基因,需要进一步的功能表征.
结论:
- 这项研究证明了古老的伪氨酸 (PM) 生物合成中的关键酶的细菌起源,挑战了以前的进化假设.
- 这一发现模糊了跨域细胞壁结构的共同起源和融合进化之间的界限.
- 这些已识别的基因为了解古老细胞壁生物学和开发新型抗古老策略提供了新的目标.
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