患有刺激性肠综合征的患者表现出内源逆转录病毒和SETDB1的异常表达
Pier-Angelo Tovo1, Davide Giuseppe Ribaldone2, Gian Paolo Caviglia2
1Department of Public Health and Pediatric Sciences, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Cells
|February 12, 2025
概括
人体内源逆转录病毒 (HERVs) 和SET域,分叉1 (SETDB1) 的过度表达可能会导致刺激性肠综合征 (IBS). 这项研究研究了IBS患者的HERV和SETDB1RNA水平,在大多数情况下发现了显著的上调.
科学领域:
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 刺激性肠综合征 (IBS) 是一种普遍存在的胃肠疾病,病因不明.
- 人体内源逆转录病毒 (HERVs) 是古代病毒感染的遗留物,构成了人类基因组的很大一部分,它们的异常表达与炎症状况有关.
- TRIM28和SETDB1在逆转录病毒转录,表观遗传调节和免疫系统调节中的作用已经确立,但它们在IBS中的参与仍然未被探索.
研究的目的:
- 与健康对照组相比,研究IBS患者外周血液中特定HERV和调节蛋白TRIM28和SETDB1的表达水平.
- 探索HERV和SETDB1表达和IBS严重程度之间的潜在相关性.
主要方法:
- 使用定量实时PCR (塔克曼试验) 来测量HERV-H-pol,HERV-K-pol,HERV-W-pol,syncytin 1 (SYN1),SYN2,HERV-W-env,TRIM28和SETDB1.1的RNA水平.
- 从37名IBS患者和年龄相匹配的健康对照组中采集了外周血液样本.
主要成果:
- 与健康对照组相比,IBS患者观察到HERV-H-pol,HERV-K-pol和SYN1的RNA水平显著更高,对SYN2和HERV-W-env具有边界意义.
- 在IBS患者中,SETDB1RNA水平显著升高,而TRIM28水平与对照人群相当.
- 与轻度或中度症状患者相比,患有严重IBS的患者表现出明显更高的SETDB1上调.
结论:
- 这些发现表明,某些HERV和SETDB1的过度表达可能在IBS的发病过程中起作用.
- 这些结果为探索针对HERVs和SETDB1在IBS管理中的新型治疗策略开辟了道路.
相关概念视频
01:17Irritable Bowel Syndrome I: Introduction
231
Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
231
01:30Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation
116
Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation
Irritable Bowel Syndrome (IBS) is classified into subtypes based on the predominant bowel habits as determined by the Bristol Stool Form Scale (BSFS). The subtypes are:
Irritable Bowel Syndrome (IBS) is classified into subtypes based on the predominant bowel habits as determined by the Bristol Stool Form Scale (BSFS). The subtypes are:
116
01:17Chronic Bowel Disorders: Introduction
384
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
384
01:17Drugs for Treatment of Diarrhea-Predominant IBS
140
Diarrhea-predominant irritable bowel syndrome (IBS-D) is a subtype of IBS characterized primarily by frequent, loose, or watery stools, abdominal pain, and abdominal discomfort. Therapeutic approaches to managing IBS-D include dietary changes, stress management techniques, and pharmaceutical interventions.
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
140
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
126
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
126
03:18Non-LTR Retrotransposons
11.3K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.3K