在冷-解后,不同排精者的精子质量特征在野猪之间存在差异
Leyland Fraser1, Łukasz Zasiadczyk1, Marzena Mogielnicka-Brzozowska1
1Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Cells
|February 12, 2025
概括
富含精子的部分 (SRF) 比整个射精 (WE) 显示出更好的冷生存,特别是在精子结能力差的野猪中. 野猪的变性显著影响冷-解后的精子质量.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
背景情况:
- 猪精液冷保存对于遗传保存至关重要.
- 在野猪中,精子质量和冷性存在变化.
- 富含精子的部分 (SRF) 和整个射精 (WE) 可能在冷生存方面有所不同.
研究的目的:
- 调查冷解后SRF和WE的精子质量特征的野猪对野猪的变化.
- 为了比较来自SRF和WE的精子的冷生存,在具有良好的 (GSF) 和糟糕的 (PSF) 精子冷能力的野猪中.
- 评估精子来源 (SRF与WE) 对解后精子参数的影响.
主要方法:
- 分析了精子运动性 (CASA),线粒体功能,膜完整性和DNA碎片化.
- 猪被分为GSF (>30%解后的总运动率) 或PSF (<30%解后的总运动率).
- 主要成分分析 (PCA1) 评估了GSF和PSF组之间的差异.
主要成果:
- PCA1解释了GSF和PSF组之间的~75%的差异,验证了解后的总运动率 (TMOT) 作为分类标准.
- 在SRF和WE的大多数解后精子参数之间观察到正相关性.
- 在SRF中,解后的参数之间的关系比WEs更强.
- 来自SRF的FT精子在PSF猪中显示了增强的TMOT,VSL和VAP.
- 与WE相比,SRF的线粒体功能,膜完整性和DNA碎片化在SRF中优越,特别是在PSF猪中.
结论:
- 单个野猪的变化显著影响冷解 (FT) 精子质量.
- 与WE相比,SRF提供了优越的冷生存,特别是在精液结能力差的野猪中.
- 在SRF和WE之间,GSF野猪的结潜力没有显著差异,这凸显了野猪变化的重要性.
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