使用非动物定义方法解决急性毒性危险分类的挑战
Donna S Macmillan1, Pravin Ambure2, Vicente Aranda2
1Humane Society International, Washington, DC, 20037, United States/now at International Collaboration on Cosmetic Safety, NY, USA.
Environmental toxicology and chemistry
|February 12, 2025
概括
一种新定义的方法 (DA) 使用计算模型和体外数据来评估鱼类的急性毒性,减少动物试验. 该方法根据全球协调系统 (GHS) 准确地分类化学危险.
科学领域:
- 生态毒理学 生态毒理学
- 计算毒理学计算毒理学
- 在体外毒理学中
背景情况:
- 鱼类急性毒性评估传统上依赖于体内试验.
- 出于道德和效率的原因,越来越需要减少在毒理学研究中使用动物.
- 开发定义方法 (DA) 和综合测试和评估方法 (IATA) 等替代方法至关重要.
研究的目的:
- 开发和验证一种基于得分的新型定义方法 (DA) 来评估鱼类的急性毒性.
- 为传统的体内试验方法提供可靠的替代方案.
- 将危害分类与联合国全球协调系统 (GHS) 保持一致.
主要方法:
- 从定量结构-活动关系 (QSARs) 中整合了三个in silico预测:VEGA鱼,Fathead Minnow (KNN-IRFMN) 和USEPA ECOSAR鱼96小时LC50.
- 整合了体外RTgill-W1数据.
- 开发一个基于分数的系统,用于分类输出.
主要成果:
- 开发的DA整合了in silico和in vitro数据.
- 该方法产生了与GHS框架一致的分类输出 (急性类别1,2,3或未分类).
- 确定DA的整体准确率为80%.
结论:
- 基于得分的新型DA提供了一个可靠和准确的替代传统的活体急性鱼类毒性测试.
- 这种方法支持在生态毒理危害评估中减少和取代动物试验.
- 符合GHS的分类促进了标准化的危险沟通.
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