在一次注射中同时进行非向代谢组和向暴露组分析的新方法
Pengwei Guan1,2,3, Yuting Wang1,2,3, Tiantian Chen1,2,3
1State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Analytical chemistry
|February 12, 2025
概括
这项研究引入了一种使用Zeno MRMHR技术同时进行代谢组和暴露组分析的新方法. 这种统一的方法减少了样本消耗和代谢组-暴露组全相关性研究 (mEWAS) 的时间.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 环境健康 环境健康
背景情况:
- 代谢学和暴露学对于理解人类健康至关重要.
- 目前的代谢组-暴露组广泛关联研究 (mEWAS) 需要单独分析,增加时间和样本需求.
- 高分辨率质谱法 (HRMS) 和三重四极联质谱法通常使用,但对组合分析有局限性.
研究的目的:
- 开发一种新的,统一的方法,在单次注射中同时进行非向代谢组和向暴露组分析.
- 为了利用Zeno MRMHR技术在mEWAS中提高灵敏度和效率.
- 为了验证该方法对内源代谢产物和外源化合物的性能.
主要方法:
- 采用Zeno MRMHR技术,同时进行高分辨率质谱 (HRMS) 和多重反应监测 (MRM) 采集.
- 使用外源标准验证该方法,评估一天内和一天内可重复性 (RSD <20%).
- 分析了90%以上的代谢特征,RSD<20%,并确定了广泛的量化范围 (LLOQ:0.125 ng/mL,HLOQ:2.51000 ng/mL).
主要成果:
- 在MRM模式下对外源性化合物的信号响应与HRMS模式下代谢物的信号响应取得了可比性.
- 证明了统一方法的高可重复性和广泛的量化范围.
- 成功地将该方法应用于2型糖尿病患者队列,以确定血清风险因素和代谢组-暴露组关联.
结论:
- 开发的统一方法可以在单次注射中同时进行非向代谢组和向暴露组分析.
- 这种新的方法为mEWAS研究带来了重大进步,减少了资源需求.
- 该方法为研究代谢物和人类健康中的暴露之间的复杂相互作用提供了一个强大的新工具.
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