LncRNA UCA1通过miRNA-4498/AKT3通路调节青诱导的AKI中的免疫微环境
Peng Hongjun1, Lydia Mukanhaire2, Liu Zhen1
1Department of Pediatrics, Nanjing Drum Tower Hospital, Affiliated hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China.
PloS one
|February 12, 2025
概括
长非编码RNA UCA1通过海绵miR-4498和上调AKT3.regulating促进急性损伤 (AKI) 的炎症. 抑制UCA1可能为西斯胺诱导的AKI提供治疗策略.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 长非编码RNAs (lncRNAs) 在急性损伤 (AKI) 中起着至关重要的作用.
- 在西斯胺诱导的AKI中,lncRNAs的特定机制需要进一步阐明.
研究的目的:
- 为了研究 lncRNA UCA1 在西斯普拉丁诱导的 AKI 中的作用和机制.
- 探索 AKI 中涉及 lncRNA UCA1,miR-4498 和 AKT3 的调节途径.
主要方法:
- 实时定量PCR用于评估cisplatin诱导的AKI小鼠模型中的lncRNA UCA1表达.
- 通过shRNA介导的lncRNA UCA1和共同培养系统的淘汰,以评估其对炎症和亡的影响.
- 双露西法酶记者测定证实了针对AKT3.3的lncRNA UCA1和miR-4498之间的相互作用.
- 流细胞计和ELISA分析炎症标志物和细胞亡.
主要成果:
- 在西斯普拉丁诱导的AKI中,lncRNA UCA1显著过度表达.
- 抑制lncRNA UCA1减少了西斯胺诱导的炎症和管状上皮细胞 (TEC) 亡.
- lncRNA UCA1 作为 miR-4498 的分子海绵,导致 AKT3 的上调.
- 通过抑制miR-4498或过度表达AKT3.3,可以逆转UCA1敲击的抗炎作用.
结论:
- lncRNA UCA1通过miR-4498/AKT3轴在西斯普拉丁诱导的AKI中促进炎症和TEC亡.
- 向lncRNA UCA1为AKI治疗提供了一个潜在的治疗途径.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:15Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
03:03mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K