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Updated: May 28, 2025

Glycopeptide Capture for Cell Surface Proteomics
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由PNGaseF生成的N-Glycans在气相中添加到上

Valentina Rangel-Angarita1, Joann Chongsaritsinsuk1, Keira E Mahoney1

  • 1Department of Chemistry, Yale University, New Haven, Connecticut 06511, United States.

Journal of the American Society for Mass Spectrometry
|February 12, 2025
PubMed
概括

通过PNGaseF的消化,释放出N-甘氨酸,通过与一起形成添加物来干扰O-甘氨酸蛋白质组分析. 使用分子量切断过器去除这些自由甘氨酸可显著改善O-糖的检测和覆盖范围.

关键词:
N-甘氨酸的含量是多少的 PNG 阶段 阶段添加材料的添加材料是什么葡萄糖蛋白质组学药物质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量这是一个很好的例子. mucinomics.

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