相关实验视频

Updated: May 28, 2025

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

SCRaMbLE:一种用于基因组操纵的多功能工具

Li Cheng¹, Jielin Li^1,2, Yaojun Chen¹

¹Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Physiology (Bethesda, Md.)

|February 12, 2025

概括

通过LoxP介导进化的合成染色体重组和修改 (SCRaMbLE) 是诱导基因组重组的强大工具. 这份审查详细介绍了SCRaMbLELE的细节.

科学领域:

遗传学遗传学是一个
合成生物学合成生物学
进化生物学进化生物学

背景情况:

基因组重组对于遗传多样性和进化至关重要.
需要新的基因组操纵工具来研究这些变异.
通过LoxP介导进化 (SCRaMbLE) 进行合成染色体重组和修改是一个关键的工具.

研究的目的:

提供SCRaMbLE系统优化的全面概述.
讨论影响SCRaMbLE性能的因素.
突出SCRaMbLE在各种生物研究中的应用.

主要方法:

使用由loxPsym站点介导的位点特异性重组.
在酵母细胞中诱导向的染色体重排.
审查SCRaMbLE的最新进展和研究结果.

主要成果:

SCRaMbLE可以实现路径工程和表型改进.
该系统促进了基因组最小化和基因型与表型关系剖析.
优化策略提高了SCRaMbLE的性能.

结论:

SCRaMbLE是研究基因组重组的多功能工具.
它为基因工程和进化研究提供了显著的优势.
潜在的应用范围超出了酵母遗传学的范围.

更多相关视频

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

相关概念视频

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...