嵌入在水凝中的过氧化酶类铜合碳点,用于刺激响应细菌生物膜清除和伤口愈合
Lin Li1, Yuhui Wang2, Shixu Hu1
1Cixi Biomedical Research Institute, Wenzhou Medical University, Ningbo 315302, PR China; Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology & Engineering (NIMTE), Chinese Academy of Sciences (CAS), Ningbo 315201, PR China.
Acta biomaterialia
|February 12, 2025
概括
铜碳点 (CuCD1) 通过在酸性生物膜微环境中产生基激素,有效地消除细菌生物膜. 这一创新为开发适应微环境的抗菌纳米剂用于伤口愈合提供了新的战略.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 传染性疾病 传染性疾病
背景情况:
- 由于药物透率差,细菌生物膜对抗菌药物开发构成重大挑战.
- 微环境响应模仿性过氧化酶 (POD) 是有前途的,但通常需要外部的H2O2或酸.
- 开发能够穿透生物膜并利用当地的微环境的药剂至关重要.
研究的目的:
- 为了合成和表征用于对抗细菌生物膜的新型POD类铜合碳点 (CuCD1).
- 研究CuCD1在对生物膜微环境的反应中对生物膜透和抗菌功效的研究.
- 为了评估CuCD1载荷的水凝的体内性能,用于伤口愈合应用.
主要方法:
- 简单的微波辅助碳化用于CuCD1合成.
- 关于 CuCD1 的尺寸 (< 5 nm),电荷 (正),和 POD 类活动的表征.
- 在体外评估CuCD1的抗菌活性和生物膜消除能力.
- 在体内评估CuCD1-CMCS-ODEX水凝用于伤口愈合和原沉积.
主要成果:
- 超小的,正电荷的CuCD1表现出极好的生物膜透性.
- 在酸性生物膜微环境中,CuCD1有效地从内源H2O2中产生基,导致细菌死亡.
- 与对照组相比,载有CuCD1的CMCS-ODEX水凝表现出优越的抗菌性能,加速伤口愈合,并促进了原的配置.
结论:
- CuCD1代表了一种新型的微环境响应抗菌纳米剂类,对细菌生物膜具有很高的疗效.
- CuCD1的简单合成和内在特性为克服生物膜相关感染提供了一个有希望的策略.
- 复合液凝配方突出了CuCD1在实际治疗应用中的潜力,特别是在伤口管理中.
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