相关实验视频

Updated: May 28, 2025

Author Spotlight: Understanding Cytokine-Induced Cell Death in Intestinal Epithelial Cells Using Human Organoids

Author Spotlight: Understanding Cytokine-Induced Cell Death in Intestinal Epithelial Cells Using Human Organoids

Published on: August 2, 2024

调查Terminalia chebula的影响,一个功能不足的水果,对口腔状细胞癌:探索细胞死亡机制

Soibam Thoithoisana Devi¹, Vimi Kshetrimayum², Rameshwari Heisnam²

¹Institute of Bioresources and Sustainable Development (An Autonomous Institute Under the Department of Biotechnology, Govt. of India) Takyelpat, Imphal- 795001, Manipur, India; Department of Zoology, Manipur University (MU), Imphal- 795003, Manipur, India.

Journal of ethnopharmacology

|February 12, 2025

概括

特米纳利亚切布拉果实提取物显示出对抗口腔癌的潜力. 乙酸乙烯分离物 (EAF) 对口腔状细胞癌 (OSCC) 细胞表现出显著的细胞毒性和诱导亡的作用,与西斯普拉丁结合时具有协同作用.

科学领域:

植物化学和药理学
癌症研究癌症研究
自然产品化学化学

背景情况:

(Terminalia chebula,TYH) 传统上用于口腔健康,并具有抗癌性质.
它对抗口腔状细胞癌 (OSCC) 的潜力仍未得到充分探索.
这项研究调查了TYH提取物及其分离物对OSCC的治疗疗效.

研究的目的:

评估Terminalia chebula水合酒精提取物 (TYH) 和其部分在SCC9口腔癌细胞上的细胞毒性和抗增殖作用.
在OSCC治疗中,评估乙酸乙酸 (EAF) 与西斯 (CP) 的协同效应.
在EAF中使用LC-ESI-QTOF-MS.识别生物活性植物成分.

主要方法:

量化了抗氧化活性,总含量 (TPC) 和黄含量 (TFC).
用MTT,克隆基因和细胞迁移试验评估了细胞毒性,增殖和迁移.
通过流动细胞计,DAPI/Giemsa染色和西部斑点分析了亡,ROS水平和蛋白质表达.

主要成果:

乙酸乙基 (EAF) 分数表现出强大的抗氧化活性,高TPC和TFC.
在SCC9细胞中,EAF表现出显著的剂量依赖性细胞毒性,抑制的增殖和迁移,以及诱导的亡.
EAF与西斯普拉丁显示出协同效应,LC-ESI-QTOF-MS确定了具有抗癌性能的和胆素.

结论:

特米纳利亚切布拉 (Terminalia chebula) 的水醇提取物,特别是其乙烯酸盐 (EAF) 分数,显示出对口腔状细胞癌 (OSCC) 的显著治疗潜力.
EAF诱导了亡,并与西斯普拉丁表现出协同效应,突出了其作为抗癌剂的前景.

关键词:

这是一种抗癌药物.抗氧化剂是一种抗氧化剂.细胞灭亡 (apoptosis) 是一种死亡的过程.乙烯酸乙烯分量 (EAF) 是一种口腔状细胞癌 (OSCC) 是一种切布拉的终点 (Terminalia chebula) 是一个大脑的终点.

更多相关视频

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Published on: April 20, 2018

相关概念视频

Cancer Therapies

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...