转子子活动在塑造cis调节元素在全基因组重复后的演变中的作用
Øystein Monsen1, Lars Grønvold1, Alex Datsomor1
1Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, 1432 Ås, Norway.
Genome research
|February 12, 2025
概括
全基因组重复 (WGD) 可以增加可移植元素 (TE) 活性,塑造基因调节. 大西洋鱼的数据显示,TE-CREs (TE-derived cis-regulatory elements) 在WGD之后进化,影响了基因网络.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 全基因组重复 (WGDs) 可以增加可移植元素 (TE) 活性,可能影响基因组进化.
- 可转移元素可以演变为cis调节元素 (TE-CREs),影响基因调节.
- 在WGD之后的监管演变中,TEs在监管演变中的作用尚未完全被理解.
研究的目的:
- 为了研究鱼 WGD 后的 TE 活动如何影响大西洋鱼的 CRE 演变.
- 识别和描述TE-CREs及其对基因调节网络演化的贡献.
主要方法:
- 使用肝脏和大脑组织的染色质可访问性数据识别了55,080个假定的TE-CREs.
- 用MPRA实验对监管活动的回归元和DNA元素进行比较分析.
- 同表达分析以推断TE驱动的基因调节网络演变.
主要成果:
- 复元是TE-CREs的主要来源,并且显示出更高的监管活动.
- 一小部分TE子家族 (16%) 占TE-CREs的很大一部分 (46%),其中许多人在WGD之前活跃.
- 来自与WGD相关的TE活性,特别是DTT (Tc1-Mariner) DNA元素的TE-CREs得到了丰富.
- 关于TE驱动的基因调控网络演变的证据,包括DTT元素在WGD周围活跃.
结论:
- TE活动在WGDs之后的基因组调节演变中发挥着重要作用.
- 这种进化可以通过放松选择或强烈选择来重新校准基因表达发生.
- 在基因组翻倍后的过渡期内活跃的TEs是监管创新的关键贡献者.
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