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在FoundationOne CDx癌症基因组分析试验中,对影响质量检查状态的因素进行现实数据分析

Hiroaki Niitsu¹, Hikaru Nakahara^1,2, Katsunari Ishida³

¹Department of Clinical and Molecular Genetics, Hiroshima University Hospital, Hiroshima, Japan.

Scientific reports

|February 12, 2025

概括

瘤纯度对于成功的综合基因组分析 (CGP) 测试至关重要. 这项研究发现,在甲固定嵌 (FFPE) 标本中瘤核的低百分比显著影响了CGP质量检查.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
病理学病理学病理学

背景情况:

全面基因组分析 (CGP) 对于个性化癌症治疗至关重要.
基于组织的CGP依赖于甲固定嵌 (FFPE) 标本.
低质量的FPE样本可能导致CGP测试失败.

研究的目的:

通过使用现实数据,识别影响CGP测试质量检查状态的因素.
确定影响FFPE标本中CGP成功的最重要因素.

主要方法:

这是一项大型的多机构研究,涉及日本广岛的1,204名参与者.
分析用于FoundationOne CDx CGP测试质量的真实数据.
对包括FFPE标本质量和癌症类型在内的因素进行统计调查.

主要成果:

瘤核的低百分比,长期FFPE块储存和胰腺癌是合格状态的独立风险因素.
瘤核的百分比对CGP质量检查状态的影响最大.
储存时间和胰腺癌对CGP质量产生了轻微影响.

结论:

瘤纯度是成功进行综合基因组分析的最关键因素.
建议在FFPE标本中至少有35%的瘤核,以获得最佳的CGP提交.
优化FFPE标本质量,特别是瘤纯度,对于可靠的基因组测试至关重要.

关键词:

癌症基因组分析癌症基因组分析基金会一个 CDx 质量检查质量检查固体瘤是一种固体瘤.

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