在Acinetobacter baumannii的菌体尾部纤维/尖端蛋白中发现功能域的发现,结构特征和进化分析
Shenshen Liu1, Tao Lei2, Yujing Tan1
1School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, China.
BMC microbiology
|February 12, 2025
概括
耐多药性Acinetobacter baumannii感染是一个越来越大的威胁. 菌体,特别是它们的尾部纤维和尖端蛋白,通过向细菌细胞壁和受体,有助于菌体治疗的发展,显示出治疗的希望.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- 全球多抗药性Acinetobacter baumannii感染的发病率不断上升,这给医疗保健带来了关键的挑战.
- 菌体治疗是治疗A. baumannii感染的常规抗生素的一个有希望的替代方案.
- 菌体尾部纤维和尖端蛋白质是通过脱聚合酶活性对宿主识别和细菌细胞壁降解的关键.
研究的目的:
- 为了识别和描述Acinetobacter baumannii菌体尾部纤维和尖端蛋白质内的功能域.
- 研究这些域之间的关系,它们的脱聚合酶或受体结合功能,以及特定的菌体.
- 为工程菌体提供基础,提高对A. baumannii的治疗疗效.
主要方法:
- 使用InterPro和AlphaFold对204个A. baumannii菌体中的313个尾部纤维和尖端蛋白的生物信息分析3.3.
- 脱聚合酶相关域的识别和分类 (Pectin lyase-like域,phage_tailspike_middle域,Transglycosidases域,SGNH酸酶域).
- 受体结合域 (pyocin_knob域,G3DSA:2.60.40.3940,新型Obo-β-sandwich结构) 的表征及其结构特征.
主要成果:
- 确定了32个功能域,包括四个与脱聚合酶相关的域 (PLD,PTMD,TGD,SHD),主要存在于Autographiviridae家族菌体 (Friunavirus属) 中.
- 素酶类域 (PLD) 显示出高变异性,但在特定的基因系内保留了区域.
- 确定了四种类型的pyocin_knob域 (PKD) 和其他受体结合域 (G3DSA:2.60.40.3940,Obo-β-sandwich),与Obolenskvirus属和Autographiviridae/Straboviridae家族相关,在受体相互作用方面表现出结构多样性.
结论:
- 这项研究阐明了A. baumannii菌体属与它们的尾巴蛋白的功能域之间的联系,突出了结构功能相关性.
- 已识别的功能域及其特征为菌体与宿主相互作用提供了洞察力.
- 这些发现可以指导菌体及其蛋白质的向工程,以改善A. baumannii感染的菌体治疗.
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