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短链脂肪酸对3T3-L1脂肪细胞adipokine分泌和代谢功能的剂量依赖性影响

Ala Alzubi¹, Hannah X Glowacki¹, Jessie L Burns¹

¹Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.

|February 13, 2025

概括

短链脂肪酸 (SCFA) 根据类型和度不同影响脂肪细胞功能. 较高的SCFA度 (1毫米) 降低了炎症性阿迪波金,但效果各不相同,酸和丁酸显示出更广泛的抗炎作用.

科学领域:

代谢科学是一种代谢科学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

碳水化合物和蛋白质的微生物发酵产生短链脂肪酸 (SCFA).
这种SCFA会影响脂细胞的脂蛋白分泌和代谢功能,可能减轻与肥胖相关的脂肪组织功能障碍.
单个SCFA的特定作用和最佳度仍然不清楚.

研究的目的:

为了研究单个SCFA对脂细胞脂蛋白分泌的剂量依赖作用.
为了确定SCFA对脂肪细胞代谢功能的影响,在炎症背景下.

主要方法:

成熟的3T3-L1脂肪细胞用脂聚糖 (LPS) 进行治疗,以模仿炎症状况.
个别的SCFA (乙酸,酸,丁酸) 用于24小时的不同度 (0.25毫米,0.5毫米,1毫米)
测量了阿迪波金分泌,细胞内蛋白质表达 (STAT3,NFκB) 和葡萄糖吸收.

主要成果:

在1mM时,所有SCFA都降低了勒素,IL-6和IL-1β的分泌;和丁酸盐也降低了MCP-1/CCL2和MIP-1α/CCL3.
乙酸在1mM时增加了RANTES/CCL5,而酸和丁酸则降低了它. 只有酸降低了MCP-3/CCL7在1毫米的速度.
和丁酸盐在0.5毫米的水平下降IL-6和IL-1β;较低的度对阿迪波金分泌没有显著影响.
在1毫米的SCFAs和0.5毫米的propionate/butyrate降低了酸化STAT3.3.
和丁酸盐在1毫米,丁酸盐在0.5毫米,减少化NFκB p65.5.
没有观察到基础或胰岛素刺激葡萄糖摄取量的显著差异.

结论:

个别的SCFA对LPS刺激的脂肪细胞功能表现出明显的剂量依赖性影响.
酸和丁酸等特定的SCFA在较高度下表现出强大的抗炎作用.
需要进一步的研究来阐明SCFA在代谢健康中的精确机制和治疗潜力.

关键词:

乙酸乙烯的使用方法脂肪细胞 (adipocytes) 是一种脂肪细胞.亚迪波基因 (Adipokine) 是一种可靠的药物.黄油酸盐黄油酸盐是一种这是一种炎症炎症炎症炎症.胰岛素刺激的葡萄糖吸收.脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.酸 propionate 的使用方法短链脂肪酸短链脂肪酸

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