在小鼠精子生成中的代谢模式的表征及其在人类的临床影响
Jiachen Wang1, Mengqi Chen1, Ying Yao1
1State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China.
International journal of molecular sciences
|February 13, 2025
概括
鉴定了精子发育过程中的代谢变化,包括甲基化减少和能量代谢改变. 在阿佐精子症中氨基酸和能量代谢的异常表明男性不孕症的潜在生物标志物.
科学领域:
- 生殖生物学 生殖生物学
- 代谢学 代谢学 代谢学
- 男人不孕症研究研究
背景情况:
- 精子生成涉及复杂的代谢适应,但这些机制的理解很少.
- 以前的研究集中在遗传和细胞方面,忽视了代谢途径.
研究的目的:
- 为了全面描述小鼠精子生成期间的代谢.
- 为了确定胚胎和体细胞之间的代谢差异.
- 探索人类亚精子症中代谢资料的临床相关性.
主要方法:
- 使用STA-PUT技术分离小鼠精子生成细胞 (包丁精子细胞,圆精子细胞,延长精子细胞).
- 对胚胎和体细胞进行非向的代谢分析.
- 来自亚精子个体的人类精液等离子体的代谢分析.
主要成果:
- 在精子生成过程中观察到显著的代谢转变,包括降低甲基化代谢物和增加糖解/TCA循环中间体在延长的精子.
- 生殖细胞和体细胞 (莱迪格,塞尔托利) 之间的明显的代谢概况,特别是在类固醇生成和脂质代谢中.
- 人类精样本显示氨酸,氨酸和氨酸的减少,途径分析表明核酸,氨基酸和能量代谢的干扰.
结论:
- 建立了精子生成的详细代谢地图.
- 代谢变化,特别是氨基酸和能量通路的变化,与男性不孕症有关,并且可以作为阿佐精子症的诊断生物标志物.
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