相关实验视频

Updated: Jul 28, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
09:56

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

3.7K

从益生菌细菌中分离细胞外囊泡的简化方法及其表征

Harshal Sawant1, Ji Bihl1, Alip Borthakur1,2

  • 1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.

International journal of molecular sciences
|February 13, 2025
PubMed
概括

研究人员开发了一种标准化的协议,用于从益生菌中分离细胞外囊泡 (EVs). 超离心产生了比降水更多的EV,而Escherichia coli Nissle的释放量超过了Lactobacillus acidophilus的释放量.

关键词:
埃舍里奇亚大肠杆菌 尼斯尔乳酸菌酸性菌类的乳酸菌.纳米粒子跟踪分析分析降水 降水 降水 降水 降水益生菌的细胞外囊泡.通过超离心法进行超离心.

更多相关视频

Isolation and Characterization of Cyanobacterial Extracellular Vesicles08:44

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

5.5K
Author Spotlight: Accelerating Research on Bacterial Extracellular Vesicles Separation and Heterogeneity06:39

Author Spotlight: Accelerating Research on Bacterial Extracellular Vesicles Separation and Heterogeneity

Published on: September 1, 2023

2.4K

相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Differential Staining Technique01:26

Differential Staining Technique

Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...