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血清和血miRNA表达水平在突然的感觉神经听力损失

Desmond A Nunez^1,2,3, Reyhaneh Abgoon^1,2, Printha Wijesinghe^1,2,4

¹Division of Otolaryngology, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1M9, Canada.

International journal of molecular sciences

|February 13, 2025

概括

这项研究发现,突然神经感官听力损失 (SSNHL) 患者的血清和血之间的microRNA (miRNA) 表达水平没有显著差异. 这两种体液都同样适合识别潜在的SSNHL生物标志物.

科学领域:

生物化学生物化学
遗传学是一个遗传学.
耳鼻喉科耳鼻喉科耳鼻喉科

背景情况:

突发感应神经听力损失 (SSNHL) 是一种异常疾病,在急性血清中识别了微RNA (miRNA) 差异表达.
在血清和血中都发现了miRNAs,但它们在反映内耳疾病变化的比较实用性尚不清楚.

研究的目的:

为了比较SSNHL的成年患者的血清和血miRNA表达水平.
确定最佳的生物流体,用于研究miRNA生物标志物SSNHL.

主要方法:

从17名SSNHL患者的血清和血样本中提取和逆转录总RNA.
使用定量实时PCR分析miRNA表达水平,将hsa-miR-191-5p作为正常化控制.
威尔科克森签名级别测试用于对miRNA表达差异的统计分析.

主要成果:

在血清和血之间,在特定miRNAs (miR-128-3p,miR-132-3p,miR-375-3p,miR-590-5p,miR-30a-3p,miR-140-3p,miR-186-5p和miR-195-5p) 的表达水平上没有发现显著差异.
使用delta Ct方法来计算miRNA表达水平.

结论:

血和血清都同样适用于调查与SSNHL相关的潜在miRNA生物标志物.
这一发现支持在未来的SSNHL诊断或预后研究中使用生物流体.

关键词:

这是一个微型RNA.血等离子体是什么?血液中的血清是血清.突然的感觉神经神经听力损失.

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Hearing

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When we hear a sound, our nervous system is detecting sound waves—pressure waves of mechanical energy traveling through a medium. The frequency of the wave is perceived as pitch, while the amplitude is perceived as loudness.

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...