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揭示了在周围植入物健康和疾病中的抵抗性景观
Lucinda J Bessa1, Conceição Egas2,3,4, João Botelho1
1Egas Moniz Center for Interdisciplinary Research (CiiEM), Egas Moniz School of Health & Science, 2829-511 Caparica, Almada, Portugal.
Journal of clinical medicine
|February 13, 2025
概括
抗生素耐药性基因在唾液中比在周围植入物生物膜中更为多样化. 在健康和近植入炎生物膜之间没有发现抗性基因的显著差异.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 口腔健康 口腔健康
背景情况:
- 人口微生物组含有抗生素耐药性基因 (ARG).
- 下周植入物生物膜是ARG的研究不足的储存库.
- 了解ARG分布对于管理与植入物相关的感染至关重要.
研究的目的:
- 调查ARG在唾液和下周围植入物生物膜中的流行和分布.
- 为了比较健康和周 implantitis条件之间的ARG概况.
- 为了确定口腔环境中ARG的分类起源.
主要方法:
- 从序列读取档案 (SRA) 中检索了100个元基因组数据集.
- 分析了从具有健康植入物和受围植入炎影响的植入物个体的样本.
- 使用ABRicate用于ARG识别和PlasmidFinder用于等离子体检测.
主要成果:
- 在89个元基因组中检测到13个类别的抗生素中55种不同的ARG.
- 麦克罗利德 - 林科萨米德 - 链条蛋白,四环素,β-乳酸盐和诺抗性基因是最常见的.
- 唾液样本显示ARG多样性明显高于下生物膜;健康生物膜和近植入炎生物膜之间没有显著差异.
结论:
- 在健康和周植入炎下膜生物膜之间,耐药性概况没有显著差异.
- 与下生物膜相比,唾液表现出更高的ARG的丰度和多样性.
- 需要进一步研究ARG在周围植入物中的传播和影响.
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