相关实验视频

Updated: May 28, 2025

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

DEAPR:差异表达和路径排名工具表明NRAS和NRAS突变在THP-1细胞中具有不同的影响

Susan K Rathe¹, Jeremy P White¹, Zohar Sachs^1,2

¹Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.

|February 13, 2025

概括

在急性髓性白血病 (AML) 中,不同的NRAS G12突变显著改变基因表达和先天免疫路径. 了解这些特定的NRAS突变可以指导定制的AML治疗策略.

科学领域:

在瘤学瘤学.
遗传学遗传学是一个
生物信息学是一种生物信息学.

背景情况:

NRAS突变发生在10%的急性髓性白血病 (AML) 患者中,通常发生在编码子12处.
人们对特定的NRAS G12突变如何影响癌细胞活性知之甚少.

研究的目的:

研究NRAS突变对AML基因表达和细胞通路的显著影响.
为了比较AML THP-1细胞与NRAS突变和修饰的THP-1细胞之间的基因表达特征.

主要方法:

使用了一种新的生物信息技术,差异表达和路径排名 (DEAPR).
在AML THP-1细胞中与NRAS突变对抗B11细胞的基因表达的比较 (NRAS-null THP-1衍生与可诱导NRAS).

主要成果:

确定了1456个独特的NRAS突变的差异表达 (DE) 基因和585个特定于NRAS突变的DE基因.
天生的免疫系统途径在两个突变特异性基因列表中都很突出.
卡尔蛋白蛋白基因 (S100A8,S100A9) 显示与NRAS突变状态相关的差异调节.

结论:

DEAPR策略揭示了NRAS突变之间的显著差异.
研究结果表明,不同的NRAS突变可能需要在AML中采用不同的治疗方法.

关键词:

发生NRAS突变的NRAS突变有关RNA测序的RNA测序这是一种calprotectin.不同的表达方式,不同的表达方式.这种白血病是白血病.路径分析路径分析

更多相关视频

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

相关概念视频

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...