在白色干奶酪中的生物氨基
Leona Buňková1, Jakub Riemel1, Khatantuul Purevdorj1
1Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlin, nám. T. G. Masaryka 5555, 760 01 Zlin, Czech Republic.
Foods (Basel, Switzerland)
|February 13, 2025
概括
在白色盐水奶酪中发现了氨酸和精氨酸等生物氨基,其中一些含量超过毒性水平. 监测乳酸细菌等特定微生物是减少奶酪中这些化合物的关键.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 生物氨基酸 (BAs) 可以通过微生物脱碳活化活动在食物中积累.
- 食品中BA的高度可能对敏感个体构成毒理风险.
研究的目的:
- 综合分析白盐奶酪中的生物氨基含量.
- 确定生物原氨酸的微生物生产者,并评估它们对积累的贡献.
主要方法:
- 在奶酪样本中分析生物性氨基 (泰拉胺,精氨酸,胺,乙胺,精氨酸).
- 检测和识别具有脱碳酶活性的微生物分离物.
- 微生物数量,乳酸细菌和生物氨基酸水平之间的相关性分析.
主要成果:
- 在20个样本中检测到生物氨基;胺和精氨酸是最丰富的.
- 在三个feta奶酪样本中,胺度超过100mg/kg.
- 25%的奶酪的总生物氨基度高于100毫克/公斤,这是毒理学上令人担忧的水平.
- 在94个分离物中发现了脱酶活性,包括*Levilactobacillus brevis*和*Enterococcus durans*.
- 观察到高微生物数量,乳酸细菌和生物氨基酸生产之间存在相关性.
结论:
- 特定的生物氨基,特别是氨酸和精氨酸,存在于白色盐水奶酪中,含量可能令人担忧.
- 微生物,特别是乳酸细菌,如Levilactobacillus brevis和Enterococcus durans,在生物氨基的形成中发挥着重要作用.
- 监测微生物种群对于减轻奶酪生产中的生物氨基风险和确保食品安全至关重要.
相关概念视频
Amines: Introduction
4.2K
Amines are organic derivatives of ammonia. They are formed by replacing one or more ammonia protons with alkyl or aryl groups. Depending upon the number of organyl groups bonded to nitrogen, amines are classified as primary, secondary, or tertiary. Primary amines have one organyl group attached to the nitrogen atom, while secondary and tertiary amines have two and three organyl groups attached to the nitrogen atom, respectively.
4.2K
Preparation of Amines: Reductive Amination of Aldehydes and Ketones
2.7K
Carbonyl compounds and primary amines undergo reductive amination first to produce imines, followed by secondary amines in the same reaction mixture, using selective reducing agents like sodium cyanoborohydride or sodium triacetoxyborohydride. Reductive amination produces different degrees of substitution of amines depending on the starting amine substrate.
2.7K
Amines to Amides: Acylation of Amines
2.4K
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
2.4K
Preparation of Amines: Reduction of Amides and Nitriles
2.4K
Nitriles can be reduced to primary amines using reducing agents like lithium aluminum hydride or catalytic hydrogenation. The reduction introduces an amino group with an extra carbon in the skeleton. Nitriles are formed from the reaction between alkyl halides and sodium cyanide through the SN2 mechanism. Primary alkyl halides are the preferred substrates to prepare nitriles.
Amides can be reduced to primary, secondary, and tertiary amines using catalytic hydrogenation, active metals like Fe,...
Amides can be reduced to primary, secondary, and tertiary amines using catalytic hydrogenation, active metals like Fe,...
2.4K
Physical Properties of Amines
3.0K
Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
3.0K
Preparation of 1° Amines: Azide Synthesis
3.8K
Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
3.8K


