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综合的Omics分析揭示了冷诱导的代谢重编程和Dendrobium officinale中的替代分离

Xinqiao Zhan^1,2, Zhangqun Li², Minxia Pang³

¹Institute of Biotechnology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang University, Hangzhou 310058, China.

Plants (Basel, Switzerland)

|February 13, 2025

概括

丹德罗比亚公办植物 (Dendrobium officinale) 是一种植物.

科学领域:

植物科学植物科学
分子生物学分子生物学
代谢学代谢学代谢学

背景情况:

经济上重要的兰花Dendrobium officinale对寒冷压力表现出敏感性.
了解寒冷压力分子和代谢机制是提高寒冷耐受性的关键.
现有的研究缺乏对D. officinale寒冷应激反应的全面数据集.

研究的目的:

为D. officinale.构建一个全面的寒冷应激反应数据集.
在不同寒冷压力条件下描述D. officinale的监管环境.
阐明寒冷耐受性所涉及的分子和代谢适应.

主要方法:

构建一个全面的冷应激反应数据集.
对基因表达的分析,包括甘氨酸代谢和碳水化合物代谢.
在冷处理 (FT) 和冷回收 (FR) 过程中脂丰度概况.
替代拼接 (AS) 分析和差异丰富的拼接体基因的识别.
识别调节拼接因子的无处不在蛋白质.

主要成果:

甘氨酸代谢基因 (Dca003913,Dca022726) 对于寒冷和干旱的适应至关重要,独立于荷尔蒙或真菌刺激.
碳水化合物代谢在受伤的细胞中表现出动态变化和荷尔蒙反应.
与FR相比,FT中的脂丰度增加,表明对寒冷强度的代谢适应.
替代拼接分析揭示了368个差异丰富的拼接 (DAS) 基因,在拼接体路径中显著丰富.
三种无处不在蛋白质 (PKU64802,XP_020672210,PKU75555) 被确定为拼接因子的调节者,在冷应力下升调.

结论:

代谢重编程和RNA剪接是D. officinale.寒冷适应的关键组成部分.
一个复杂的分子网络涉及代谢途径和替代拼接在冷应激期间被激活.
结果为开发策略提供了洞察力,以改善这种重要的兰花物种的耐寒性.

关键词:

丹德罗比亚公办植物 (Dendrobium officinale) 是一种植物.替代性拼接是一种替代性的拼接.寒冷的压力是冷的压力.甘氨酸的代谢甘氨酸的代谢无处不在的化

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