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在低温和微弱光线下对茄子中安托西亚宁生物合成基因的比较转录基因分析

Baoying Shen¹, Hongqi Wu¹, Xinxin Xie²

¹Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Plants (Basel, Switzerland)

|February 13, 2025

概括

低温和低温/弱光相结合会增加茄子的安东素,而仅仅弱光会降低它们. 这与安托素生物合成途径中的关键基因调节有关.

科学领域:

植物生理学植物生理学
生物化学生物化学
分子生物学分子生物学

背景情况:

温度和光强度等环境因素显著影响植物生长和质量.
对茄子皮肤颜色负责的安托西亚宁对水果质量至关重要,对环境线索敏感.
了解调节在压力下安托氨酸生物合成的分子机制对于作物改善至关重要.

研究的目的:

为了研究低温,弱光和它们的组合对茄子抗生素含量的影响.
为了阐明在这些压力条件下参与氨酸生物合成的分子途径和基因调节.
确定关键的基因和转录因子,与茄子皮中改变的安东素积累相关.

主要方法:

茄子接受了四种处理:低温 (LT),弱光 (WL),低温和弱光 (LW) 组合,以及控制 (CK).
在不同的茄子部分中量化了安托西亚宁含量.
使用RNA测序对茄子皮进行了转录组分析,随后进行了KEGG通路分析和皮尔森相关性分析.

主要成果:

与CK相比,在LT和LW治疗中,安托西亚宁含量增加,但在WL治疗中减少.
在KEGG的分析中,发现了烯胺生物合成,黄胺生物合成和氨酸生物合成的关键途径.
在压力下,发现了安托西亚宁含量和基因 (例如,SmPAL,SmCHS,DFR,3GT) 和转录因子 (MYB,bHLH,AP2/ERF) 之间存在显著的相关性.

结论:

低温和低温/弱光压力组合积极调节茄子皮中的安东素积累.
氨酸的生物合成受到氨酸和氨酸路径的显著影响.
3GT基因的向下调节在 LT 和 LW 应激下增加安托素含量方面起着至关重要的作用.

关键词:

安托西安尼尼 (anthocyanin) 是一种茄子 (Solanum melongena L.) 是一种植物.转录因子的转录因子转录组 (transcriptome) 是一个转录组.

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