相关实验视频

Updated: May 7, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

基于血液的Yili马的全基因组甲基化分析赛前和赛后

Jianwen Wang^1,2, Wanlu Ren^1,2, Zexu Li¹

¹College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China.

Animals : an open access journal from MDPI

|February 13, 2025

概括

这项研究揭示了Yili马在赛后的DNA甲基化变化,确定了与马的运动表现和运动反应相关的关键基因和表观遗传途径.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
马类基因组学马类基因组学
运动生理学运动生理学

背景情况:

了解体育表现中的表观遗传调节对于优化马匹训练和健康至关重要.
伊利马是赛马中的重要品种,但它们的运动诱导的表观遗传修饰仍然在很大程度上未被探索.

研究的目的:

为了研究5000米比赛前后的Yili马的全基因组DNA甲基化差异.
确定与赛车表现相关的差异甲基基因 (DMGs),并探索运动的潜在表观遗传机制.
为了确定马匹运动表现的候选基因.

主要方法:

整个基因组二硫酸盐测序 (WGBS) 用于在赛前和赛后从Yili马的血液样本上.
分析包括识别差异甲基化区域 (DMR) 和DMG.
对DMG进行了基因本体学 (GO) 和基因和基因组京都百科全书 (KEGG) 路径丰富分析.

主要成果:

确定了大量的差异甲基化CG,CHG和CHH区域.
在基因体和促进器区域中发现了大量的DMG.
丰富分析突出了PI3K-Akt信号传递等途径,并确定了潜在的候选基因 (例如,IFNAR2,FGF4,DGKH).

结论:

运动显著改变了Yili马的DNA甲基化模式.
表观遗传修饰,特别是与激酶活性和信号通路相关的基因,都与马的运动表现有关.
这项研究为了解马的运动表观遗传学和改进比赛策略提供了基础.

关键词:

通过DNA甲基化.伊利利马 (Yili Horse) 是一匹马,可以骑马.不同地甲基化基因的基因.赛车比赛表现的表现.进行全基因组测序.

更多相关视频

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

相关概念视频

Pedigree Analysis

Pedigree Analysis

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...