在五次饮酒后,乙烯基葡萄化物和乙烯基硫酸盐的血液和血清度比
Matthias Bantle1, Annette Thierauf-Emberger2,3, Alexandra Schröck4
1Institute of Forensic Medicine, University of Bern, Bern 3008, Switzerland.
Journal of analytical toxicology
|February 13, 2025
概括
这项研究确定了乙烯基葡萄糖化物 (EtG) 和乙烯硫酸盐 (EtS) 酒精生物标志物的血液与血清比率. 结果显示EtG主要分布在血清中,而EtS在全血中度更高.
科学领域:
- 法医毒理学 法医毒理学
- 生物标记分析 生物标记分析
- 药理动力学 药理动力学
背景情况:
- 乙基葡萄化物 (EtG) 和乙基硫酸盐 (EtS) 是关键的酒精生物标志物,主要研究在尿液中.
- 关于全血中EtG和EtS度的数据有限,因此需要研究血液与血清的比率,用于法医应用.
研究的目的:
- 为了研究饮酒后血与血清EtG和EtS的比率.
- 在涉及血清和全血样本的法医毒理病例中,为解释EtG和EtS水平提供关键数据.
主要方法:
- 在5次饮酒后10天内从11名健康参与者同时采集血清和全血样本.
- 使用液体染色学-并联质谱法 (LC-MS/MS) 分析EtG和EtS.
- 通过气色谱-火焰电离检测 (GC-FID) 和基于酒精脱酶的方法量化乙醇.
主要成果:
- 消费后4-7小时EtG和ETS达到峰值.
- 血液与血清的平均比率为EtG的0.58 (范围:0.38-0.73) 和EtS的0.81 (范围:0.61-0.92).
- 血清和全血度之间观察到很高的相关性;没有发现血液与血清比率的时间或度依赖性.
结论:
- EtG主要分布在血清中,而EtS在全血中度更高.
- 确定的血与血清比值对于酒精生物标志物的法医解释非常有价值.
- 个体排泄档案没有显示EtG或EtS曲线进展中的规律性.
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