过度表达OsDUF868.12可以提高大米的盐分耐受性
Hao Chen1, Jiale Wan1, Jiali Zhu1
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, China.
Frontiers in plant science
|February 13, 2025
概括
米基因OsDUF868.12通过改善氧化应激防御和调节应激反应基因来提高盐分耐受性. 过度表达增加了生存率,而淘汰却减少了它们,突出了它在非生物压力管理中的关键作用.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 土壤盐度是一个主要的非生物压力,限制了全球的作物生产率.
- 不知功能的域868 (DUF868) 蛋白家族存在于大米中,但其在压力反应中的作用在很大程度上是未知的.
研究的目的:
- 为了克隆和功能性地描述大米基因OsDUF868.12.
- 为了阐明OsDUF868.12在米对盐应激反应中的作用.
主要方法:
- 基因克隆和功能表征.
- 用RT-qPCR进行基因表达分析.
- 亚细胞局部化研究.
- 在盐应激下过度表达和淘汰米线的表型分析.
- 生理和生化分析. 生理和生化分析.
主要成果:
- OsDUF868.12的表达显著受到低温和盐应激的影响.
- OsDUF868.12 局部存在于细胞膜中.
- 过度表达OsDUF868.12提高了大米的盐耐受性和生存率.
- 淘汰赛的OsDUF868.12减少了盐分的耐受性.
- 过度表达改善了氧化应激防御和高调盐应激相关基因.
结论:
- OsDUF868.12在提高大米对盐应激的耐受性方面发挥着至关重要的作用.
- 过度表达OsDUF868.12在分子水平的盐应激条件下具有显著的优势.
- 这项研究提供了关于DUF868家族在植物非生物应激反应中的作用的见解.
更多相关视频
08:27Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
4.3K
09:43Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
2.1K
相关概念视频
02:04Regulation of Transpiration by Stomata
32.4K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
32.4K
01:57Adaptations that Reduce Water Loss
29.0K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
29.0K
02:41Responses to Drought and Flooding
12.4K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
12.4K
02:02Responses to Salt Stress
15.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
15.1K
01:20Tonicity in Plants
44.3K
Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
44.3K