在平虫中搜索和功能注释多域PLA2家族蛋白质
M E Bocharnikova1, I I Turnaev2, D A Afonnikov1
1Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, Russia Kurchatov Genomic Center of ICG SB RAS, Novosibirsk, Russia.
这项研究系统地确定了平虫中的脂酶A2 (PLA2) 基因,揭示了寄生虫中的基因损失和与宿主相互作用相关的重复. 这些发现揭示了寄生虫平虫中PLA2的演变.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 寄生虫学的寄生虫学
背景情况:
- 脂酶A2 (PLA2) 酶是参与细胞过程的关键酸酶.
- 虽然在模型生物中得到了很好的研究,但在像平虫这样的非模型生物中,PLA2s仍然是鲜为人知的.
- 一些PLA2s涉及致癌和寄生虫与宿主相互作用.
研究的目的:
- 系统地识别和比较分析平虫基因组中的脂酶A2 (PLA2) 基因序列.
- 了解自由生活和寄生虫平虫中PLA2基因的进化模式.
- 调查PLA2基因进化和寄生虫与宿主相互作用之间的潜在联系.
主要方法:
- 在44种平虫物种中全基因组搜索PLA2序列 (2种自由生活,42种寄生虫).
- 基于正基因基因组和蛋白质域结构的比较分析.
- 保存和分类特定的PLA2基因家族的识别.
主要成果:
- 在已知的13种动物PLA2类型中,有12种在平虫中被鉴定出来,分为11个正统组.
- 独立于的PLA2,G8和G15类型在大多数平虫种群中都存在.
- 寄生虫平虫表现出显著的基因损失,而自由生活物种显示基因重复;分泌的PLA2群是Digenea特有的,并在opisthorchids中重复.
结论:
- 这项研究为平虫中PLA2序列的首次系统识别提供了帮助.
- 在平虫中,PLA2的演变的特征是遗传损失,这是寄生虫基因组的典型特征.
- 寄生虫中的特定类型基因重复和PLA2s的损失可能对宿主生物相互作用至关重要.
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