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Antonio Suma^1,2, Cristian Micheletti³

¹Dipartimento di Fisica, Università di Bari and INFN, Sezione di Bari, Bari, Italy.

|February 13, 2025

概括

由于拓摩擦和纠,DNA结减缓了纳米孔在高力下解锁的速度. 这种复杂性影响单分子实验和体内DNA交易.

科学领域:

聚合物物理聚合物物理
生物物理学的生物物理.
纳米技术纳米技术

背景情况:

通过纳米孔进行DNA转移对于聚合物物理学和生物过程至关重要.
在生物系统中,DNA结是很常见的,它会影响DNA的动态.

研究的目的:

调查DNA结对纳米孔解锁动态的影响.
了解节点拓如何影响在不同力下解压.

主要方法:

分子动力学模拟的模拟.
粗粒度DNA建模粗粒度DNA建模
对结结的DNA解压的分析.

主要成果:

节点对在低力下解锁的影响很小.
高强力导致结结的DNA的缓慢和异质解.
障碍是由于拓摩擦和与未拉链的DNA纠而产生的.

结论:

DNA拓学显著复杂化了纳米孔在更高的力量下解锁.
这些发现与解释纳米孔实验和模拟体内DNA交易有关.

关键词:

它们是DNA DNA DNA DNA.结的结,结的结.纳米孔转移转移的方法拓摩擦的拓学摩擦打开拉链的时间

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DNA Topoisomerases

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...