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病毒诱导的基因沉默同时利用植物和昆虫的"吸引和杀死"特征来管理黄龙

Nabil Killiny¹, Yasser Nehela², Subhas Hajeri³

¹Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, United States.

Horticulture research

|February 13, 2025

概括

研究人员对类植物进行了改造,以吸引和杀死亚洲类虫,这是黄龙 (HLB) 的载体. 这种新的吸引和杀死方法使用病毒诱导的基因沉默来创造吸引和减少虫种群的植物,为类绿化疾病提供潜在的治疗方法.

科学领域:

植物病理学植物病理学
昆虫学昆虫学是一门学科.
分子生物学分子生物学

背景情况:

黄龙 (HLB) 是一种无法治愈的破坏性类疾病,造成全球经济重大损失.
亚洲类植物 (Diaphorina citri) 是HLB的主要载体,使其控制对疾病管理至关重要.

研究的目的:

用病毒诱导的基因沉默来设计类植物,以吸引和杀死Diaphorina citri.
开发一种新的"吸引和杀死"战略,通过将类作物转化为载体陷和致命剂来管理HLB.

主要方法:

改造了传染性悲伤病毒 (CTV-T36) 克隆,以表达三个截断的基因 (CTV-tAwd-tWnt-tPDS).
工程病毒产生小干扰RNA (siRNAs),向D. citri在吸引力 (PDS) 和飞行/生存 (DcAwd,DcWnt) 中的基因.
在两代工程植物中评估了D. citri的死亡率,生育能力,生育能力,寿命和翅膀形态.

主要成果:

具有CTV-tAwd-tWnt-tPDS的工程植物由于改变了植物线索,优先吸引D. citri.
向的siRNAs导致了D. citri.的翅膀形,降低了生存率,降低了生育能力和生育能力.
草的寿命缩短了20多天,工程植物的花液证实了siRNA的存在,并减少了成年人的生存率.

结论:

病毒诱导的基因沉默可以设计类植物作为吸引剂和致命剂对HLB载体D. citri.
这项研究提供了一种新的吸引和杀死策略的概念证明,将作物转化为超吸引力的载体槽.
这种方法有望开发可持续的综合性害虫防治解决方案,用于HLB和其他媒介传播的植物疾病.

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