一个无体 (+) RNA 亚多卡里病毒被dRNA 病毒托管,以粒子,cDNA 和dRNA 的形式具有传染性
Muhammad Fadli1, Sakae Hisano1, Guy Novoa2
1Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.
Journal of virology
|February 13, 2025
概括
像AfSV2这样的无囊病毒 (capsidless yadokariviruses) 劫持伴侣病毒的囊体. 这项研究表明,AfSV2以粒子,cDNA或dsRNA的形式具有传染性,扩大了已知的yadokarivirus合作伙伴.
科学领域:
- 病毒学 病毒学
- 菌类学 菌类学是指菌类学.
背景情况:
- 无体的亚多卡里病毒 (Yadokarivirales序列) 使用辅助病毒体.
- 亚多卡病毒拥有 (+) RNA 基因组,在真菌中发现.
- 之前的报道已经发现了来自*Aspergillus foetidus*的yadokarivirus.
研究的目的:
- 为了识别 *Aspergillus foetidus* 中的yadokarivirus AfSV2 的辅助病毒.
- 为了描述AfSV2.2的传染性形式.
- 为了扩大已知的yadokarivirus伙伴病毒的范围.
主要方法:
- 从真菌菌株中隔离和净化病毒.
- 原生体感染病毒颗粒,cDNA和dsRNA.
- 用核酶和蛋白酶处理以确定传染性实体的组成.
- 病毒RNA指导RNA聚合酶的遗传分析.
主要成果:
- AfSV1 (维多利病毒) 被确定为AfSV2.2的辅助病毒.
- AfSV2通过病毒颗粒,全长cDNA和纯化复制形式 (RF) dsRNA.表现出感染力.
- 感染需要AfSV1的存在才能发生AfSV2的cDNA转化.
- RNase III 治疗消除了 AfSV2 RF dsRNA 的感染性,这表明了 dsRNA 的基因组.
结论:
- AfSV2是一种具有dSRNA基因组的独特传染实体.
- 这项研究将雅多卡病毒的已知伴侣扩展到*假ototiviridae*家族.
- 研究结果提供了关于病毒与宿主相互作用的演变的见解.
相关概念视频
Retrovirus Life Cycles
45.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.6K
Viral Structure
61.6K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.6K
Retroviruses
12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
What are Viruses?
113.6K
Overview
113.6K
RNA Interference
25.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.9K
Rous Sarcoma Virus (RSV) and Cancer
4.9K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
4.9K


