揭示易于电离和坚固的离子物种:在ESI-MS分析中提高电离效率的门户
Benjamin B Warnes1, Jasmine Chihabi1, Jeffrey M Manthorpe1
1Department of Chemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
Journal of the American Society for Mass Spectrometry
|February 13, 2025
概括
新的离子增强负离子电喷射电离化质谱法,用于分析具有挑战性的分子. 四氧化[3,5-bis(三甲基) ]酸盐和 bis(非-1-) 硫胺酸盐显示出最佳的电离效率和稳定性,用于化学衍生.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 有机化学 有机化学
背景情况:
- 电喷离子质谱 (ESI-MS) 对于分析低挥发性分子至关重要.
- 低电离效率 (IE) 对ESI-MS分析构成挑战.
- 化学衍生物化 (CD) 改善了IE,但负离子CD方法很少.
研究的目的:
- 在ESI-MS.中开发和评估用于负离子化学衍生物化的新离子.
- 为了识别具有高电离效率,稳定性和诊断联质谱仪行为的离子.
- 为了解决负离子分析的CD技术的差异.
主要方法:
- 根据相对IE,碰撞诱导解离稳定性和协同MS行为评估了27个离子.
- 与二甲基硫酸盐内部标准相比较的离子.
- 评估了各种溶剂的IE,并评估了阴离子对阴离子性能的影响.
主要成果:
- 高离子显示增强了IE.
- 四氧化[3,5-bis(三甲) ]酸盐 (23) 和 bis(非-1-butan) 硫胺酸 (25) 显示最佳IE (332% ± 25% 和 939% ± 92%) 和稳定性.
- 与常见的离子相比,离子25表现出显著的信号增强 (136%181%).
结论:
- 阴离子23和25在ESI-MS中对负离子化学衍生物化非常有效.
- 这些离子为分析具有挑战性的分子提供了更好的电离效率和稳定性.
- 开发的离子代表了负极性ESI-MS工作流程的重大进步.
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