相关实验视频

Updated: Jun 19, 2026

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

戈安红树林酵母:一种治疗酶L-asparaginase的来源

Aabha Marathe¹, Lakshangy Charya²

¹Microbiology Discipline, School of Biological Sciences and Biotechnology, Goa University, Goa, India.

Folia microbiologica

|February 13, 2025

概括

来自印度红树林沉积物的热带酵母显示出产生酶L-asparaginase的潜力. 这项研究优化了酵母酵母产生的条件Cyberlindnera GULAMMS8.8.

科学领域:

微生物学微生物学
酶学是一种酶学.
生物技术是生物技术.

背景情况:

红树林生态系统有各种适应波动的环境条件的微生物.
来自这些息地的热带酵母是具有临床意义的酶的潜在来源,例如L-asparaginase.
L-阿斯巴拉金酶是癌症治疗中通过耗尽L-阿斯巴拉金而至关重要的酶.

研究的目的:

从印度红树林沉积物中分离和选热带酵母,用于生产L-asparaginase.
为了识别具有高L-asparaginase活性的酵母分离物.
为了优化物理和营养参数,以提高L-asparaginase的产量.

主要方法:

从印度戈阿的红树林沉积物中分离出14种热带酵母菌株.
使用指示染料的快速板测试对L-阿斯巴拉金酶活性进行查.
使用标准分类学方法识别最活跃的分离物.
优化化时间,pH值,温度,碳源和源.

主要成果:

在14种酵母分离物中,有5种表现出L-asparaginase活性.
鉴定出具有最高活性的分离物属于Cyberlindnera (GULAMMS8) 属.
酶生产的最佳条件包括48小时的化时间,pH值7.0,28°C,葡萄糖作为碳来源,L-阿斯巴拉丁作为唯一的来源.
在优化条件下,L-asparaginase活性翻了一番,潜伏期缩短了一半.

结论:

热带红树林酵母,特别是Cyberlindnera GULAMMS8,是L-asparaginase的一个有希望的来源.
物理和营养因素显著影响了这种酵母中的L-阿斯巴拉基纳酶的产生.
这项研究为进一步研究来自红树林衍生的酵母L-asparaginase的潜在治疗应用提供了基础.

关键词:

化疗剂化疗剂化疗剂在L-asparaginase的使用中.红树林森林是红树林的森林.热带酵母热带酵母是一种热带酵母.

更多相关视频

In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease

In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease

Published on: December 20, 2017

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

相关概念视频

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Production of Antibiotics

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...