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由SNORD80引导的2'-O-甲基化稳定了lncRNA GAS5,以调节细胞应激反应

Liang Xu¹, Xiao Hong Zhao¹, Yuan Yuan Zhang¹

¹School of Biomedical Sciences and Pharmacy, The University of Newcastle, Newcastle, NSW 2308, Australia.

Proceedings of the National Academy of Sciences of the United States of America

|February 13, 2025

概括

小核RNA SNORD80指导甲基化以稳定长非编码RNA GAS5.5. 这种相互作用创建了一个压力诱导的调节循环,增强GAS5表达.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
基因规则基因规则

背景情况:

长非编码RNA (lncRNA) GAS5基因含有内部子,它们是多个C/D盒小核RNA (snoRNA) 的宿主.
GAS5与其宿主snoRNAs,特别是SNORD80之间的功能关系在很大程度上仍未被描述.

研究的目的:

研究GAS5及其宿主snoRNA之间的调控和功能关系,重点关注SNORD80.
阐明SNORD80在GAS5表达的转录后调节中的作用.

主要方法:

对细胞应激反应中的基因表达的分析.
研究RNA-RNA相互作用和RNA修饰 (2'-O-甲基化).
对SNORD80和纤维拉林的基因淘汰和过度表达研究.

主要成果:

SNORD80的表达与GAS5的表达平行,并且在细胞应激下被共同调节.
在GAS5上,SNORD80在特定位置引导2'-O-甲基化,增强其稳定性和表达.
通过SNORD80介导的甲基化依赖于纤维拉林,盒子C/DsnoRNP的甲基转移酶.

结论:

通过引导2'-O-甲基化,SNORD80在稳定GAS5方面发挥着至关重要的作用.
在GAS5位点存在一个前调节循环,SNORD80在应对细胞应激时稳定GAS5.
这项研究揭示了控制lncRNA表达的新型转录后机制,并突出了snoRNAs和lncRNAs之间的相互作用.

关键词:

通过2′-O-甲基化.气体5 气体5 是一个气体.在SNORD80中,我们可以使用SNORD80 在ncRNA中,我们可以斯诺RNA 斯诺RNA 是一种

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