使用Cutibacterium acnes ZW-1进行可扩展的酸生产:洞察基质和pH驱动的碳流
Zeyu Wang1, Jun Chen2, María C Veiga3
1Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310015, China; Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology - Centro Interdisciplinar de Química y Biología (CICA), BIOENGIN group, University of La Coruña (UDC), E-15008 La Coruña, Spain.
The Science of the total environment
|February 13, 2025
概括
研究人员利用一种耐酸的Cutibacterium acnes菌株优化了酸 (PA) 生产. 在最佳条件下,PA的产量为64.84毫米,扩展达到83.31毫米,支持工业生物生产.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 昆虫细菌 (Cutibacterium acnes) 是一种常见的人类皮肤细菌.
- 酸 (PA) 有各种工业应用.
- 优化微生物PA生产对于可持续制造至关重要.
研究的目的:
- 隔离和表征一种耐酸的Cutibacterium acnes菌株,用于增强酸 (PA) 生产.
- 确定最佳的基质和pH条件,以最大限度地提高PA产量.
- 评估大规模PA生物生产的可行性.
主要方法:
- 从人体皮肤中分离出耐酸的Cutibacterium acnes ZW-1.
- 在不同的乳酸 (LA) 到酸 (AA) 比例和pH值下进行发酵研究.
- 分析PA度,碳流量,电子效率和酶活性.
- 在自动化生物反应器中进行扩大规模的验证.
主要成果:
- 在LA:AA摩尔比率为7:1和pH值为6.5.5的情况下,可以实现最佳的PA产量 (64.84mM).
- 观察到高的PA碳流量 (59%) 和电子效率 (79%).
- 生物反应器的扩展使PA产量增加到83.31 mM,其速率为1.066 g·L−1·d−1.1.
- 轻微酸性pH (<6.5) 有利于碳流向PA,但受到生物质的限制.
结论:
- 耐酸的Cutibacterium acnes ZW-1菌株显示了PA生物生产的巨大潜力.
- 优化的发酵条件和扩大规模的战略提高了PA的产量和生产率.
- 这项研究为微生物PA合成的工业应用提供了基础.
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