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一种基于BR-CD的光复合材料,用于GSH的选择性检测

Yuqi Wan¹, Shuqi Li¹, Jiahua Wang²

¹State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.

|February 13, 2025

概括

使用蓝色和红色发射碳点 (BR-CDs) 的新的光比策略可以选择性地检测出无氨酸干扰的谷氨 (GSH). 这种方法在食品样本中准确量化GSH,显示出高回收率.

科学领域:

分析化学分析化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

生物乙醇,如谷氨 (GSH),在细胞过程中至关重要.
精确检测GSH对于了解生物功能和疾病状态至关重要.
氨酸 (Cys) 通常会干扰基于光的生物醇检测方法.

研究的目的:

开发一种选择性和敏感的光检测策略来检测谷氨 (GSH).
为了克服氨酸 (Cys) 在生物醇检测中的干扰.
为了创建一个双排放的碳点复合材料,用于定量GSH分析.

主要方法:

由蓝色发射 (B-CD) 和红色发射碳点 (R-CD) 组成的复合材料的制造.
基于复合材料对GSH.反应的光比策略 (FL440/FL620) 的开发.
在各种食品矩阵 (番茄,梨,土豆) 中验证方法.

主要成果:

在存在GSH时,BR-CDs复合物表现出红色排放的减少和蓝色排放的恢复.
观察到光比与GSH的线性反应在30500μmol/L的范围内.
在GSH检测方面,获得了5.27μmol/L的低检测极限 (LOD).
在食品样本中获得了高恢复率 (94.36%112.02%) 和低RSD% (<0.41%).

结论:

开发的BR-CDs复合物能够选择性和敏感地检测GSH.
光比率策略有效地减轻了囊干扰.
该方法证明了GSH在现实世界食品样本中的量化实际应用.

关键词:

生物乙醇是一种生物乙醇.碳点是一些碳点.光检测方法的光检测方法.检测GSH的检测方法遮蔽比光光遮蔽比光

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