影响气候呼吸室中乳牛平衡估计的技术因素
K Nichols1, S J J Alferink1, T Zandstra1
1Animal Nutrition Group, Wageningen University and Research, 6700 AH Wageningen, the Netherlands.
Journal of dairy science
|February 13, 2025
概括
分析方法显著影响乳牛N平衡计算. 元素分析 (EA) 提供了比Kjeldahl (KJEL) 更高的N摄入和保留估计,这表明KJEL可能低估了料N含量.
科学领域:
- 动物营养 动物营养
- 农业化学 农业化学
- 环境科学 环境科学
背景情况:
- 乳牛中精确的 (N) 平衡对于优化营养和最小化环境影响至关重要.
- 传统的N分析方法可能会在N平衡估计中引入变化.
- 气候呼吸室 (CRC) 用于测量N组件,但分析方法和样品处理可以影响结果.
研究的目的:
- 评估不同 (N) 分析方法 (Kjeldahl [KJEL]与元素分析 [EA]) 对乳牛N平衡成分的影响.
- 评估气候呼吸室 (CRC) 内样本采集和储存协议对N估计的影响.
- 确定分析变化如何影响N摄入量,牛奶N分泌量,便N分泌量,挥发性N外观和N保留量的测量.
主要方法:
- 28头荷尔斯坦-弗里西亚牛被安置在CRC中进行N平衡测量.
- 用KJEL和EA.分析了料 (玉米料,草料) 和缩剂的N含量.
- 使用这两种方法收集和分析了牛奶,和挥发性N (空气和冷凝).
- 测试了有和没有酸性保存的便样本.
主要成果:
- 元素分析 (EA) 显示,料中的N含量高于Kjeldahl (KJEL).
- 当EA分析时,摄入量较高,但牛奶和肥中的含量没有受到分析方法的显著影响.
- 与EA (36 g/d) 相比,KJEL (19 g/d) 的留量显著较低,这归因于KJEL对较低料N的分析.
- 空中N测量方法 (酸陷与NH3分析仪) 没有影响挥发性N的外观.
结论:
- 选择N分析方法,特别是料,显著影响乳牛的N摄入量和N保留估计.
- 虽然KJEL可能低估了料N,可能导致N保留的人工通货膨胀,但EA提供了更全面的N评估.
- 对于在CRC中量化挥发性N,酸陷和NH3分析仪都是合适的,采样/储存协议没有缓解过高估计问题.
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