循环微生物组DNA特征及其对预测结直肠癌患者临床病理特征的影响
Liang Ma1, Mingliang Shi2, Xue Zhang1
1Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Journal of translational medicine
|February 13, 2025
概括
结直肠癌 (CRC) 患者的周围静脉血液微生物组与健康个体不同. 血液中的特定微生物与CRC转移和进展相关,这表明它们有可能作为非侵入性生物标志物.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 大肠直肠癌 (CRC) 涉及复杂的瘤微环境,受遗传和微生物因素的影响.
- 微生物DNA可以从肠道和瘤转移到血液中,形成循环微生物群.
- 这种循环微生物组与CRC的预后和临床病理特征有关.
研究的目的:
- 为了研究CRC患者的外周静脉血液微生物组.
- 评估CRC中循环微生物组的临床意义.
- 与健康对照人群相比,在CRC患者中识别疾病特异性微生物特征.
主要方法:
- 来自29名CRC患者和10名健康对照者的外周静脉血液样本使用2bRAD-M测序进行了分析.
- 通过RT-qPCR检查了20名CRC患者的瘤组织样本,以验证血液瘤微生物相关性.
- 统计分析评估了微生物丰富度和临床特征之间的关联,包括转移和PD-L1综合阳性得分 (CPS).
主要成果:
- 总共有270种微生物物种被确定,其中Actinomycetota,Bacillota,Bacteroidota和Pseudomonadota作为主导类.
- 博西亚狼与转移阶段有显著的关联 (p=0.034).
- 结核菌,Porphyromonas pasteri和Bosea lupini与CPS相关,这表明潜在的瘤衍生的转移.
结论:
- 在CRC患者中存在一个独特的外周静脉血液微生物组.
- 血液中的特定微生物与CRC临床病理特征和疾病进展有关.
- 血液微生物组显示出作为CRC预后和治疗点的非侵入性生物标志物的潜力.
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