无膜微生理系统的简单设计可以模拟血组织屏障
Ashlyn T Young1, Halston Deal1,2, Gabrielle Rusch1,2
1Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina, Chapel Hill, 911 Oval Dr., Raleigh NC, 27695, USA.
Organs-on-a-chip
|February 14, 2025
概括
研究人员使用3D水凝支架和人体细胞开发了一种新的无膜血组织接口芯片 (BTI芯片). 这种微生理系统 (MPS) 允许细胞直接接触以进行先进的药物查和生物研究.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 组织工程是组织工程.
背景情况:
- 微生理系统 (MPS) 对于模仿组织功能在体外至关重要.
- 现有的血液-组织接口 (BTI) 模型通常依赖于膜,同质细胞或2D培养,限制了它们的生理相关性.
- 目前的BTI模型在组装和复制3D传输和直接内皮-上皮接触方面面临着挑战.
研究的目的:
- 设计一种新的BTI-on-a-chip (BTI芯片),克服当前模型的局限性.
- 开发一种无膜,单层BTI芯片设计,用于增强生理模拟.
- 为了使研究和工业能够高吞吐量生成生理学上相关的MPS.
主要方法:
- 用于in situ组织制造的使用了层状流程配置文件和可光固化的水凝支架 (凝甲基和8臂聚乙烯糖) .
- 通过紫外线照射构建了一个Y形的微流体装置,用于通过紫外线照射来快速聚合支架.
- 将内皮细胞直接播种到3D工程组织支架上,创建上皮和血脑屏障 (BBB) 模型.
主要成果:
- 成功制造了一个没有膜的BTI芯片,与内皮直接接触3D组织.
- 免疫组织化学证实了与工程组织相对应的均内皮.
- 使用光追踪剂和排泄抑制剂 (环素A) 证明了生理功能和透性.
结论:
- 开发的BTI芯片提供了一个生理学上相关的,无膜的微生理系统.
- 这种新的设计使直接的细胞与细胞相互作用和3D传输成为可能,这对于精确的体外建模至关重要.
- 该BTI芯片为药物候选查和基本生物研究提供了高通量MPS生成的便利.
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