相关实验视频

Updated: May 28, 2025

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

培养基度的阶段性变化促进了猪卵细胞的成熟in vitro

Lixiang Liu^1,2, Yu Tang^1,2, Jing Shao^1,2

¹Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.

Frontiers in cell and developmental biology

|February 14, 2025

概括

用甘氨酸优化培养基的度,可以改善猪卵细胞的成熟和发育潜力. 这项研究强调了细胞体积调节对于成功的体外卵子细胞发育的重要性.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
细胞生理学细胞生理学

背景情况:

哺乳动物胚胎对高透性条件敏感,导致发育停止.
细胞体积稳态对于成功的体外胚胎发育至关重要.
卵细胞在卵泡脱落后获得独立体积调节,但最佳成熟条件需要进一步研究.

研究的目的:

调查优化细胞体积调节是否能提高猪卵细胞的成熟和质量.
为了确定调整培养基度与甘氨酸对卵细胞成熟的影响.

主要方法:

猪卵细胞在44小时的两个阶段进行了体外成熟 (IVM).
培养基的度以阶段依赖的方式 (290mOsM为22h,然后320mOsM为22h) 用1mM的甘氨酸进行调整.
拉芬用于增加度,因为它不会被动物细胞代谢.

主要成果:

用甘氨酸调节依赖阶段的度,显著改善了猪卵细胞成熟率.
观察到卵细胞质量的提高,包括线粒体功能和分布的改善.
在颗粒细胞中发现了增加的发育潜力和有利的基因表达,以及减少的活性氧物种 (ROS) 水平.

结论:

通过阶段特定的介质度调整来优化细胞体积调节,可以增强体外卵细胞成熟.
这种方法改善了卵细胞质量和随后的胚胎发育潜力.
这些发现强调了细胞体积恒温在辅助生殖技术中的关键作用.

关键词:

细胞体积调节细胞体积调节甘氨酸 (glycine) 是一种在体外成熟的成熟.这就是度的度 (osmolarity).猪卵细胞是猪的卵细胞.

更多相关视频

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

Published on: July 8, 2020

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

相关概念视频

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...