在蒙古乌兰巴托的生牛肉和肉类加工环境中识别 Staphylococcus aureus 引起污染
Amgalanzaya Dorjgochoo1,2, Anujin Batbayar3, Altansukh Tsend-Ayush1
1Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
International journal of microbiology
|February 14, 2025
概括
黄金葡萄球菌 (S. aureus) 在26.9%的生牛肉和15.3%的肉类市场表面中被发现. 虽然甲基西林和万科米辛耐药性基因不存在,但检测到了毒性基因,强调了在肉类运输和销售中改善的卫生.
科学领域:
- 食品微生物学 食品微生物学
- 细菌污染是一种细菌污染.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 黄金葡萄球菌 (S. aureus) 是一个重要的病原体,导致抗生素耐药性增加的感染.
- 蒙古附近地区的甲素耐药金黄色细菌 (MRSA) 患病率值得注意.
- 食品的污染对公众健康构成风险.
研究的目的:
- 为了确定生牛肉和肉类市场环境中的金黄色细菌污染水平.
- 在S. aureus分离物中识别毒性和抗生素耐药性基因.
- 评估孤立的金黄色菌株的抗生素敏感性模式.
主要方法:
- 使用ISO 6888-1:2021标准收集和分析156个生牛肉样本和131个表面拭子.
- 聚合酶链反应 (PCR) 用于识别S. aureus (nucA基因) 和检测耐药性 (mecA, mecC, vanA, vanB) 和毒性基因 (海, sed, tsst, eta, 等).
- 通过Kirby-Bauer盘扩散进行抗生素敏感性测试.
主要成果:
- 在26.9%的生牛肉和15.3%的表面抹布中检测到S. aureus污染.
- 在肉类分离物中观察到对牛素,皮素和青素的高耐药性;在表面分离物中观察到牛素,四环素,亚齐胺素和克林达胺素.
- 没有发现甲基西林 (mecC) 或万科米辛 (vanA,vanB) 耐药基因. 确定了病毒性基因tsst,海,etb,eta和sed.
- 与加工区相比,在运输和销售中心发现污染率更高.
结论:
- 在研究区域的生牛肉和肉类市场被S. aureus污染.
- 病毒性基因的存在是令人担忧的,尽管没有特定的甲基西林和万科米抗性基因.
- 在肉类运输和市场上,加强卫生和处理实践至关重要,以减轻黄金菌的污染和传播.
相关概念视频
Special Staining Techniques
1.7K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.7K
Microbes in Food Production
409
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
409
Microbes in Beverage Production
306
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
306
Microbes in the Production of Fermented Foods
340
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
340
Microbial Spoilage of Food
230
Microbial food spoilage refers to the degradation of food quality resulting from the metabolic activity of microorganisms such as bacteria, yeasts, and molds. These microbes proliferate on various food substrates depending on factors such as moisture content, nutrient availability, and storage conditions, leading to undesirable sensory and structural changes.Bacteria are primary agents of spoilage in high-moisture, nutrient-dense foods like meat, milk, and vegetables. Microbial spoilage occurs...
230
Clinical Significance of Antibiotic Resistance
115
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
115


