敏感于pH值的聚甲烯酸 (聚甲烯酸) 植入刷涂层的温度响应特性,可控制细胞培养的湿透性
Ostap Lishchynskyi1,2, Svitlana Tymetska3,4, Yana Shymborska3
1Lviv Polytechnic National University, St. George's Square 2, Lviv, 79013, Ukraine. yrstecushun@ukr.net.
Journal of materials chemistry. B
|February 14, 2025
概括
聚甲酸 (PMAA) 涂料表现出对温度敏感的特性,由于出色的生物相容性,显示出生物医学应用的潜力. 然而,它们的较低的临界溶液温度 (LCST) 并没有影响蛋白质吸附或细胞形态.
科学领域:
- 聚合物科学 聚合物科学
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 聚甲酸 (PMAA) 是公认的pH响应,但它的温度响应行为是不太了解.
- 将PMAA植入表面会产生具有可调节性质的刷涂层.
研究的目的:
- 为了研究PMAA移植刷涂层的温度反应性质.
- 评估这些涂料的生物相容性和潜在的生物医学应用.
主要方法:
- 在玻璃表面通过使用甲基酸 (NaMAA) 和甲基酸 (MAA) 的表面启动原子转移基聚合 (SI-ATRP) 合成PMAA刷涂层.
- 使用水接触角度测量,湿度和厚度分析来确定较低的临界溶液温度 (LCST) 的特征涂层.
- 在PMAA涂层上评估细胞活力和皮肤纤维细胞的生长.
主要成果:
- 从MAA合成的PMAA涂层与NaMAA的涂层相比,具有较低的湿透性.
- 对于PMAA刷涂料,观察到较低的临界溶液温度 (LCST) 过渡,通过湿度和厚度的变化表明了这一点.
- 尽管之前有关于抑制细胞生长的报道,但MAA的PMAA涂层与皮肤纤维细胞具有很好的生物相容性.
- 在LCST过渡没有影响蛋白质 (BSA) 吸附或纤维细胞细胞形态.
结论:
- PMAA刷涂料具有温度响应特征,基于合成单体的独特特性.
- 来自MAA的PMAA涂层的水性增加增加了生物相容性,表明它们适合用于生物医学用途.
- 这些PMAA涂层中的LCST过渡目前没有转化为受控的蛋白质吸附或细胞行为变化.
相关概念视频
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
Stomach pH Regulation
The human body carefully regulates the internal pH of different organs to maintain homeostasis. For example, while the blood plasma maintains a neutral pH of 7, the stomach lumen has an acidic pH of 1.5 - 3.5. The low pH of stomach lumen helps kill pathogens in the food and break down complex food molecules.
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
pH Regulation in Cells
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Factors Influencing Microbial Growth: pH
Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...


