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基于双极电极的精确操纵和细胞的选择性电穿孔

Kemu Li^1,2, Yupan Wu^1,2,3,4, Yan Hu⁵

¹School of Integrated Circuits, Southeast University, Sipailou 2, Nanjing 210096, P. R. China.

Analytical chemistry

|February 14, 2025

概括

这项研究引入了一种新的双极电极方法,用于精确的单细胞操纵和电穿孔. 这种技术使得在各种细胞类型的治疗和诊断应用中可实现向的细胞内输送.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
电穿孔是一种电子穿孔.

背景情况:

对特定单细胞的细胞内传递对于向治疗和诊断至关重要.
目前的方法通常依赖于复杂的微操纵器或多步制造,限制了实际使用.

研究的目的:

开发一种用于精确操纵和单细胞选择性电穿孔的新方法.
克服现有的细胞内输送技术的局限性.

主要方法:

采用双极电极系统,通过介电泳 (DEP) 精确定位细胞.
使用来自直流 (DC) 脉冲列车的局部强化电场实现了选择性电穿孔.
采用旋转电场用于活动细胞的准和移动.

主要成果:

证明了对细胞定位和选择性电穿孔的精确控制.
成功将光染料分子和等离子体输入各种细胞类型 (酵母,K562,THP-1,293T,SNU-1).
展示了该方法在广泛的细胞类型,包括细菌的适用性.

结论:

双极电极方法提供了一种通用,高效和新的工具,用于有针对性的细胞内输送.
这种技术促进了单细胞分析,并为未来的治疗和诊断应用提供了显著的潜力.

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Electrodeposition

Electrodeposition

Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...