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克罗恩病的深度测序Lamina Propria Phagocytes识别了病原体,并与亲炎性基因表达相关
Gillian E Jacobsen1,2,3, Eddy E Gonzalez3, Payton Mendygral3
1Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, USA.
克罗恩病 (CD) 阴茎中的细胞含有诸如大肠杆菌 (E. coli) 这样的特定细菌,这些细菌在宿主中显示出炎症基因表达的增加. 这些发现揭示了CD疗法的潜在病原生物和免疫点.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 微生物组研究 微生物组研究
背景情况:
- 克罗恩氏病 (CD) 涉及对肠道微生物群的炎症反应.
- 巨细胞和树突细胞是CD炎症的关键参与者.
- 特定的肠道微生物涉及到叶CD的病变发生.
研究的目的:
- 通过使用无偏序测序来研究CD中的细胞相关微生物组.
- 为了识别可能导致CD的病原生物.
- 了解宿主对这些微生物的免疫反应.
主要方法:
- 从CD患者和对照组中收集了大肠粘膜和大肠粘膜活检.
- 隔离的自身膜细胞 (CD11b+细胞) 用于深度RNA测序.
- 进行了微生物组分类学和元转录学分析,在第二个队列中得到证实.
主要成果:
- 在CD中的叶细胞显示出较高的埃舍里希亚大肠杆菌,鲁米诺科克斯菌和Enterocloster spp.的丰度.
- 与CD相关的微生物表现出增加的脂多糖 (LPS) 生物合成途径表达.
- 具有较高病原生物负担的细胞显示出高的促炎和抗微生物基因表达.
结论:
- 在lamina propria中居住的细胞携带细菌菌株,在CD中充当病原体.
- 这些发现揭示了病原生物和免疫反应在CD病变发生过程中的作用.
- 确定了CD的潜在新治疗点.
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