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在印度尼西亚拉湾发现的棕色藻的抗氧化活性评估和使用DNA条形码cox1 BLAST的分子鉴定
Betutu Senggagau1, Manja Meyky Bond2, Suryadi Saputra2
1Research Center for Fishery, National Research and Innovation Agency, Jl. Raya Bogor KM.47, Nanggewer Mekar, West Java, Indonesia. bsenggagau@yahoo.com.
Molecular biology reports
|February 14, 2025
概括
来自印度尼西亚拉湾的棕色大藻类使用DNA条形码识别并测试了抗氧化活性. 三种物种 - - 青 (Sargassum plagiophyllum),青 (Sargassum ilicifolium) 和状 (Hormophysa cuneiformis) - - 显示出强大的抗氧化特性.
科学领域:
- 海洋生物学 海洋生物学
- 物理学的生理学
- 生物化学 生物化学
背景情况:
- 关于印度尼西亚拉湾棕色大藻的分子鉴定和抗氧化性质的研究有限.
- 这项研究通过调查当地棕色宏观藻类物种来弥补这一差距.
研究的目的:
- 通过分子方法,从拉湾南部海岸识别棕色大藻类物种.
- 评估已识别的棕色大藻类物种的抗氧化活性.
主要方法:
- 通过使用DNA条形编码与cox1基因和BLAST分析进行了分子鉴定.
- 使用DPPH自由基清除试验评估了抗氧化活性.
- 棕色藻样本从塞巴朗格海,卡利安达和南拉蒙普的佩萨瓦兰收集.
主要成果:
- 鉴定出了三种主要的棕色大藻类物种:Sargassum plagiophyllum,Sargassum ilicifolium和Hormophysa cuneiformis. 这三种主要的棕色大藻类是 Sargassum plagiophyllum,Sargassum ilicifolium和Hormophysa cuneiformis.
- 所有确定的物种都表现出显著的DPPH自由基清除活动.
- 乙醇提取物显示出强大的抗氧化潜力,其中Sargassum ilicifolium显示出最低的EC50值 (64.17 mg L-1).
结论:
- 鉴定到的棕色藻种类具有强大的抗氧化活性.
- 种类变异在测试度范围 (50-100 mg L-1) 内的抗氧化能力没有显著影响.
- 这些发现突显了当地棕色藻作为天然抗氧化剂来源的潜力.
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