菌体内溶酶LysLFP01及其抗菌活性的表征
Qiannan Wen1, Xuecheng Huang2, Wenxin Ma2
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; Inner Mongolia Academy of Science and Technology, Hohhot, Inner Mongolia 010000, China.
International journal of food microbiology
|February 14, 2025
概括
一种新的Lactobacillus菌酶,LysLFP01,显示出广泛的抗菌活性和强大的稳定性. 这种酶有效地抑制金黄色葡萄球菌生物膜,并减少生牛奶中的细菌数量,为食品安全应用提供了潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 菌体编码具有抗微生物潜力的糖酶 (酶).
- 对于来自Lactobacillus菌体的lyses存在有限的表征.
- 来自乳杆菌菌体LFP01的LysLFP01是调查的目标.
研究的目的:
- 在大肠杆菌中克隆和表达乳杆菌菌酶 (LysLFP01).
- 描述LysLFP01.01的抗菌活性,生物膜抑制和稳定性.
- 为了评估LysLFP01在原奶中的有效性,用于食品安全应用.
主要方法:
- 在大肠杆菌中LysLFP01的异质表达.
- 对抗 Staphylococcus aureus 生物膜的抗菌谱和活性的确定.
- 评估热稳定性 (4-72°C),pH耐受性 (3.0-9.0),盐耐受性.
- 在4°C的生牛奶中评估抗菌活性.
主要成果:
- LysLFP01 显示出广泛的抗菌活性,特别是在对抗格拉姆阳性细菌.
- 该酶表现出显著的热稳定性和广泛的pH活性,在高NaCl度下有效性降低.
- 莱斯LFP01有效地抑制和去除了黄金色菌生物膜,并减少了生牛奶中的细菌数量.
结论:
- 莱斯LFP01是一种强大有效的抗菌剂,具有食品安全潜力.
- 它对S. aureus生物膜的有效性和稳定性使其适用于低盐,非酸性食品环境.
- 需要进一步研究LysLFP01在对抗食物传播病原体中的应用.
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