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肠道微生物群的变化会影响DNA甲基化水平和肉肌肉组织的发展

Yibin Xu¹, Yunpeng Huang¹, Shenghua Wei¹

¹State Key Laboratory of· Livestock· and Poultry Breeding, & Lingnan Guangdong Laboratory of Agriculture, South China Agricultural University, & Guangzhou Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, PR China.

Poultry science

|February 14, 2025

概括

在中,抗生素诱导的肠道微生物群抑制减少了肌肉生长. 肠道微生物通过DNA甲基化影响肌肉发育,突出显示肠道微生物组与骨肌肉调节之间的联系.

科学领域:

动物科学动物科学
微生物学微生物学
遗传学遗传学是一个

背景情况:

肠道微生物组在宿主肌肉生长和发育中起着至关重要的作用.
肠道微生物组失调症,通常以抗生素治疗为模式,对其对黄色羽毛肉肌肉生长的影响的研究有限.
了解肠-肌肉轴对于优化家禽生产至关重要.

研究的目的:

为了研究肠道微生物组变化和Xinghua在抗生素诱导的失生症后肌肉生长之间的关系.
确定分子机制,包括基因表达和DNA甲基化,将肠道微生物群与骨肌肉发育联系起来.

主要方法:

用抗生素诱导的肠道微生物群抑制,持续8周.
16S rRNA测序用于分析肠道微生物的组成和多样性.
转录组分析 (RNA测序) 用于在肌肉组织中识别差异表达基因 (DEGs).

主要成果:

抗生素治疗显著降低了体重,肌肉重量和肠道中的微生物多样性.
观察到肠道微生物组成的显著变化,Firmicutes,Actinobacteria和Bacteroidetes减少,Proteobacteria增加.
失生症导致肌肉中的298个DEG,其中67个与骨肌肉发育相关的下调基因 (例如,MYF6,MYBPC1,METTL21C).
肠道微生物组失调影响了肌肉组织中的DNA甲基化,降低了DNMT3b和METTL21C的表达和整体DNA甲基化水平.

结论:

肠道微生物群失生症会对的肌肉生长和发育产生负面影响.
肠道微生物组通过表观遗传修饰,特别是DNA甲基化,影响骨肌肉的发育.
准肠道微生物组是提高家禽肌肉生长的潜在策略.

关键词:

通过DNA甲基化.增长和发展增长和发展我们的肠道微生物组.黄色羽毛的小黄羽的小

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