相关实验视频

Updated: May 28, 2025

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

皮层祖先种群的克隆谱系追踪和转录组学揭示了差异化潜力的维持

Danyon Harkins¹, Shawar Ali¹, Teodora Tockovska²

¹Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto. 1 King's College Circle, Toronto, ON M5S 1A8, Canada.

Stem cell reports

|February 14, 2025

概括

个别的皮层辐射质原生体 (RGP) 是多能的,在产后产生内神经元,星球细胞和寡细胞. 细胞分化潜力持续到出生后的第4天,这表明新皮层发育的种群下降模型.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
细胞生物学细胞生物学

背景情况:

产后新皮层发育涉及放射性质原始体 (RGP) 从神经发生向质发生的过渡.
了解个人RGP的血统潜力对于破译大脑发育至关重要.

研究的目的:

在产后发育过程中调查个体皮质RGP的血统潜力.
为了确定细胞命运限制和原始细胞对不同细胞类型的贡献的时间.

主要方法:

使用多层谱系追踪工具 (pbacBarcode) 来追踪单个皮质RGP.
分析了RGP对出生后皮层中各种细胞类型的贡献.

主要成果:

发现个别的皮层RGP具有多能性,在2:1:1的比率下产生嗅球内部神经元,星球细胞和寡细胞.
差异化潜力一直保持在出生后的第四天,支持人口下降模型,而不是严格的细胞命运限制.
增殖性中间细胞的群体可能有助于产生主要细胞类型.
皮层RGP在很大程度上在出生后的第三天完成了寡基细胞原始细胞创始细胞的生产.

结论:

皮层RGP在出生后早期的发育中保持了多效力,有助于多样化的神经和质谱系.
产后新皮层发育的特点是原始细胞群动力学,而不是仅仅是细胞命运限制.
通过RGPs产生的寡细胞在很大程度上是在出生后的第三天建立起来的.

关键词:

克隆追踪 (clonal tracing) 是一种可以追踪一个人的行为.发展发展发展发展发展.格利亚 (Glia) 是一个.谱系追踪谱系追踪谱系追踪新皮质新皮质的新皮质这就是scRNA-seqq.空间转录学空间转录学

更多相关视频

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Published on: August 9, 2017

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

相关概念视频

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...