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对昆虫细胞的基因和途径进行全基因组的CRISPR查,以应对异常的环境pH值

Jiasong Chang¹, Qi Li¹, Tong Zhang²

¹Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Taiyuan, China; Department of Physiology, Shanxi Medical University, Taiyuan, China.

International journal of biological macromolecules

|February 14, 2025

概括

这项研究使用全基因组的CRISPR查来识别涉及昆虫细胞对极端细胞外pH (pHe) 变化反应的基因和途径. 关键的途径,如酸氨基信号传递和mRNA监测,对于细胞抵抗异常的pHe至关重要.

科学领域:

细胞生物学细胞生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

细胞外pH值 (pHe) 受到严格调节,其偏差与各种疾病有关.
了解细胞对异常pHe的反应对于基础科学和医学都至关重要.
控制细胞适应pH压力的基因和信号通路基本上是未知的.

研究的目的:

确定涉及昆虫细胞适应极端细胞外pH的基因和途径.
为细胞对pH挑战的反应提供基因组规模的视角.
发现pH相关疾病的潜在治疗点.

主要方法:

在Bombyx mori胚胎细胞系 (BmEGCKLib) 上进行了全基因组的CRISPR查.
细胞在各种异常的细胞外pH条件 (pH 5.0,5.5,6.6,6.95) 和生理pH (6.3) 下进行培养.
基因和基因组的京都百科全书 (KEGG) 路径丰富分析对已识别的基因进行.

主要成果:

44个重叠的健身基因和24个重叠的积极选择基因在极端的pH条件下被确定.
"酸氨基醇信号系统"",mRNA监测途径"和"结合体途径"在负选择中显著丰富.
这些发现强调了细胞信号传导和mRNA质量控制在pH电阻中的重要性.

结论:

细胞信号传导和mRNA质量控制对于昆虫细胞来说至关重要,以抵抗异常的细胞外pH值.
这项研究为昆虫细胞对pH压力的反应提供了第一个基因组规模的洞察力.
鉴定出来的基因和通路为未来疾病和医学研究带来了潜力.

关键词:

细胞外的pH值整个基因组的查.昆虫的细胞是昆虫的细胞.

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