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Updated: May 28, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

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基于基质改性及其高效生物制造的高通量选方法用于糖脂类.

Yuanyi Li¹, Zhilin Liu¹, Yiming Zheng²

¹College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.

Bioresource technology

|February 14, 2025

概括

一种新的基质改性高通量选 (HTS) 方法可以促进微生物合成. 这种方法增强了脂和脂的生产,使成本有效的生物制造成为可能.

关键词:

发酵发酵是一个过程.高产品的高产品种.拉姆诺脂类是什么索福利皮德 (Sophorolipid) 是一种高度脂质.

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科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
合成生物学合成生物学

背景情况:

高通量查 (HTS) 对于改善微生物合成至关重要.
基于基因编辑的HTS在非模型微生物菌株中通常是不可行的.
开发替代HTS方法对于生物制造的进步至关重要.

研究的目的:

为非模型微生物菌株开发基质修改的HTS策略.
为了增强脂 (SLs) 和脂 (RLs) 的产生.
通过提高微生物产量,促进具有成本效益的生物制造.

主要方法:

优化的发酵介质将SL的产量提高到248g/L.
合成的2-乙酸作为脂类类似物生产的基质.
采用暗色表型选择和254nm吸收用于高产SLs菌株选,成功率为51.1%.
将HTS方法扩展到拉姆诺脂类 (RLs) 生产.
设计了一个微气泡反应堆,以提高RL的产量.

主要成果:

通过A6.菌株获得324g/L的脂 (SLs) 产量.
成功选了高产SLs菌株,成功率为51.1%.
使用工程微气泡反应器,拉姆诺脂类 (RLs) 的产量增加到70.3g/L.

结论:

基质修改的HTS策略对于像Starmerella bombicola.这样的非模型菌株有效.
这种HTS方法显著增加了微生物发酵产品的产量.
开发的方法使有价值化合物的高效和成本效益的生物制造成为可能.